The metal-responsive transcription factor-1 protein is elevated in human tumors

Yihui Shi, Khalid Amin, Barbara G. Sato, Steven J. Samuelsson, Lidia Sambucetti, Zishan A. Haroon, Keith Laderoute, Brian J. Murphy

Research output: Contribution to journalArticle

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Abstract

We previously identified metal-responsive transcription factor-1 (MTF-1) as a positive contributor to mouse fibrosarcoma growth through effects on cell survival, proliferation, tumor angiogenesis and extracellular matrix remodeling. In the present study, we investigated MTF-1 protein expression in human tissues by specific immunostaining of both normal and tumor tissue samples. Immunohistochemical (IHC) staining of a human tissue microarray (TMA), using a unique antihuman MTF-1 antibody, indicated constitutive MTF-1 expression in most normal tissues, with liver and testis displaying comparatively high levels of expression. Nevertheless, MTF-1 protein levels were found to be significantly elevated in diverse human tumor types, including breast, lung and cervical carcinomas. IHC analysis of a separate panel of fullsize tissue sections of human breast cancers, including tumor and normal adjacent, surrounding tissue, confirmed and extended the results of the TMA analysis. Taken with our previous findings, this new study suggests a role for MTF-1 in human tumor development, growth or spread. Moreover, the study suggests that MTF-1 could be a novel therapeutic target that offers the opportunity to manipulate metal or redox homeostasis in tumor cells.

Original languageEnglish (US)
Pages (from-to)469-476
Number of pages8
JournalCancer Biology and Therapy
Volume9
Issue number6
DOIs
StatePublished - Mar 15 2010

Fingerprint

Neoplasms
Proteins
Tissue Array Analysis
Breast Neoplasms
Fibrosarcoma
Human Development
Growth
transcription factor MTF-1
Oxidation-Reduction
Extracellular Matrix
Testis
Cell Survival
Homeostasis
Metals
Cell Proliferation
Staining and Labeling
Carcinoma
Lung
Antibodies
Liver

Keywords

  • Human breast carcinomas
  • Human tissue microarray
  • Human tumors
  • Immunohistochemistry
  • MTF-1

Cite this

Shi, Y., Amin, K., Sato, B. G., Samuelsson, S. J., Sambucetti, L., Haroon, Z. A., ... Murphy, B. J. (2010). The metal-responsive transcription factor-1 protein is elevated in human tumors. Cancer Biology and Therapy, 9(6), 469-476. https://doi.org/10.4161/cbt.9.6.10979

The metal-responsive transcription factor-1 protein is elevated in human tumors. / Shi, Yihui; Amin, Khalid; Sato, Barbara G.; Samuelsson, Steven J.; Sambucetti, Lidia; Haroon, Zishan A.; Laderoute, Keith; Murphy, Brian J.

In: Cancer Biology and Therapy, Vol. 9, No. 6, 15.03.2010, p. 469-476.

Research output: Contribution to journalArticle

Shi, Y, Amin, K, Sato, BG, Samuelsson, SJ, Sambucetti, L, Haroon, ZA, Laderoute, K & Murphy, BJ 2010, 'The metal-responsive transcription factor-1 protein is elevated in human tumors', Cancer Biology and Therapy, vol. 9, no. 6, pp. 469-476. https://doi.org/10.4161/cbt.9.6.10979
Shi, Yihui ; Amin, Khalid ; Sato, Barbara G. ; Samuelsson, Steven J. ; Sambucetti, Lidia ; Haroon, Zishan A. ; Laderoute, Keith ; Murphy, Brian J. / The metal-responsive transcription factor-1 protein is elevated in human tumors. In: Cancer Biology and Therapy. 2010 ; Vol. 9, No. 6. pp. 469-476.
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