The Medicago genome provides insight into the evolution of rhizobial symbioses

Nevin D Young, Frédéric Debellé, Giles E.D. Oldroyd, Rene Geurts, Steven B. Cannon, Michael K. Udvardi, Vagner A. Benedito, Klaus F.X. Mayer, Jérôme Gouzy, Heiko Schoof, Yves Van De Peer, Sebastian Proost, Douglas R. Cook, Blake C. Meyers, Manuel Spannagl, Foo Cheung, Stéphane De Mita, Vivek Krishnakumar, Heidrun Gundlach, Shiguo ZhouJoann Mudge, Arvind K. Bharti, Jeremy D. Murray, Marina A. Naoumkina, Benjamin Rosen, Kevin A Silverstein, Haibao Tang, Stephane Rombauts, Patrick X. Zhao, Peng Zhou, Valérie Barbe, Philippe Bardou, Michael Bechner, Arnaud Bellec, Anne Berger, Hélène Bergès, Shelby Bidwell, Ton Bisseling, Nathalie Choisne, Arnaud Couloux, Roxanne Denny, Shweta Deshpande, Xinbin Dai, Jeff J. Doyle, Anne Marie Dudez, Andrew D. Farmer, Stéphanie Fouteau, Carolien Franken, Chrystel Gibelin, John Gish, Steven Goldstein, Alvaro J. González, Pamela J. Green, Asis Hallab, Marijke Hartog, Axin Hua, Sean J. Humphray, Dong Hoon Jeong, Yi Jing, Anika Jöcker, Steve M. Kenton, Dong Jin Kim, Kathrin Klee, Hongshing Lai, Chunting Lang, Shaoping Lin, Simone L. MacMil, Ghislaine Magdelenat, Lucy Matthews, Jamison McCorrison, Erin L. Monaghan, Jeong Hwan Mun, Fares Z. Najar, Christine Nicholson, Céline Noirot, Majesta O'Bleness, Charles R. Paule, Julie Poulain, Florent Prion, Baifang Qin, Chunmei Qu, Ernest F. Retzel, Claire Riddle, Erika Sallet, Sylvie Samain, Nicolas Samson, Iryna Sanders, Olivier Saurat, Claude Scarpelli, Thomas Schiex, Béatrice Segurens, Andrew J. Severin, D. Janine Sherrier, Ruihua Shi, Sarah Sims, Susan R. Singer, Senjuti Sinharoy, Lieven Sterck, Agnès Viollet, Bing Bing Wang, Keqin Wang, Mingyi Wang, Xiaohong Wang, Jens Warfsmann, Jean Weissenbach, Doug D. White, Jim D. White, Graham B. Wiley, Patrick Wincker, Yanbo Xing, Limei Yang, Ziyun Yao, Fu Ying, Jixian Zhai, Liping Zhou, Antoine Zuber, Jean Dénarié, Richard A. Dixon, Gregory D. May, David C. Schwartz, Jane Rogers, Francis Quétier, Christopher D. Town, Bruce A. Roe

Research output: Contribution to journalArticlepeer-review

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Abstract

Legumes (Fabaceae or Leguminosae) are unique among cultivated plants for their ability to carry out endosymbiotic nitrogen fixation with rhizobial bacteria, a process that takes place in a specialized structure known as the nodule. Legumes belong to one of the two main groups of eurosids, the Fabidae, which includes most species capable of endosymbiotic nitrogen fixation. Legumes comprise several evolutionary lineages derived from a common ancestor 60 million years ago (Myr ago). Papilionoids are the largest clade, dating nearly to the origin of legumes and containing most cultivated species. Medicago truncatula is a long-established model for the study of legume biology. Here we describe the draft sequence of the M. truncatula euchromatin based on a recently completed BAC assembly supplemented with Illumina shotgun sequence, together capturing ∼ 94% of all M. truncatula genes. A whole-genome duplication (WGD) approximately 58 Myr ago had a major role in shaping the M. truncatula genome and thereby contributed to the evolution of endosymbiotic nitrogen fixation. Subsequent to the WGD, the M. truncatula genome experienced higher levels of rearrangement than two other sequenced legumes, Glycine max and Lotus japonicus. M. truncatula is a close relative of alfalfa (Medicago sativa), a widely cultivated crop with limited genomics tools and complex autotetraploid genetics. As such, the M. truncatula genome sequence provides significant opportunities to expand alfalfa's genomic toolbox.

Original languageEnglish (US)
Pages (from-to)520-524
Number of pages5
JournalNature
Volume480
Issue number7378
DOIs
StatePublished - Dec 22 2011

Bibliographical note

Funding Information:
FOOD-CT-2004-506223; to G.E.D.O. and J.R. from BBSRC BBS/B/11524; to F.D. and F.Q. from ANR project SEQMEDIC 2006-01122; to R.G. from the Dutch Science Organization VIDI 864.06.007, ERA-PG FP-06.038A; to Y.V.d.P. from the Belgian Federal Science Policy Office IUAP P6/25, Fund for Scientific Research Flanders, Institute for the Promotion of Innovation by Science and Technology in Flanders and Ghent University (MRP N2N); to D.R.C. from NSF IOS-0531408, IOS-0605251; to D.J.S.,B.C.M.and P.J.G.fromUSDACSREES2006-03567;toJ.Gouzyfrom‘Laboratoire d’Excellence’ (LABEX) TULIP (ANR-10-LABX-41). We also acknowledge technical support from the University of Minnesota Supercomputer Institute and thank Y. W. Nam for a BamHI BAC library used by Genoscope, S. Park and M. Accerbi for RNA isolation, T. Paape for statistical consulting, and M. Harrison for supplying myc infected and control root tissues used to make small RNA libraries.

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