The mechanism of silver staining of proteins separated by SDS polyacrylamide gel electrophoresis

E. Hempelmann, K. Krafts

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Gel based silver staining of proteins is thought to occur by selective reduction of silver ions to insoluble metallic silver at specific initiation sites in the vicinity of the protein molecules. Silver stained protein bands generally are dark brown or black with considerable variation in color intensity. The color variation has been attributed to diffractive scattering by silver grains of different sizes. Our experiments, however, demonstrate that color variation is due to the formation of silver chromate deposits that are incorporated into formalin fixed proteins. Understanding the mechanism of silver staining is essential for developing a method for protein quantification.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalBiotechnic and Histochemistry
Volume92
Issue number2
DOIs
StatePublished - Feb 17 2017

Bibliographical note

Publisher Copyright:
© 2017 The Biological Stain Commission.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • electrophoresis
  • polyacrylamide gels
  • proteins
  • quantification
  • silver stain

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