The magnetic susceptibility of reduced cytochrome P-450cam

P. M. Champion, E. Münck, P. G. Debrunner, T. H. Moss, J. D. Lipscomb, I. C. Gunsalus

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


The magnetic susceptibility of singly reduced, camphor-complexed cytochrome P-450 from Pseudomonas putida has been measured at various temperatures. Computer analysis using a spin Hamiltonian gives values of D between 15K and 25K, which agrees with a value of D = 20K found by low-field variable temperature Mössbauer spectroscopy. This result is compared with those from deoxyhemoglobin and deoxy-myoglobin.

Original languageEnglish (US)
Pages (from-to)579-582
Number of pages4
JournalBBA - Bioenergetics
Issue number3
StatePublished - Mar 20 1975

Bibliographical note

Funding Information:
high-spin iron atom in reduced P-450 has an isolated ground state oribital (with spin quintet) conducive to the spin Hamiltonian formalism, while the heme iron of deoxyhemoglobin and deoxymyoglobin might be better described Using a different approach \[1 2 \]. The authors would like to thank M.J. Na~ntvedt for laboratory assistance. This work was partially supported hy U.S. Public Health Service grant GM 16406


Dive into the research topics of 'The magnetic susceptibility of reduced cytochrome P-450cam'. Together they form a unique fingerprint.

Cite this