The magnetic susceptibility of singly reduced, camphor-complexed cytochrome P-450 from Pseudomonas putida has been measured at various temperatures. Computer analysis using a spin Hamiltonian gives values of D between 15K and 25K, which agrees with a value of D = 20K found by low-field variable temperature Mössbauer spectroscopy. This result is compared with those from deoxyhemoglobin and deoxy-myoglobin.
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high-spin iron atom in reduced P-450 has an isolated ground state oribital (with spin quintet) conducive to the spin Hamiltonian formalism, while the heme iron of deoxyhemoglobin and deoxymyoglobin might be better described Using a different approach \[1 2 \]. The authors would like to thank M.J. Na~ntvedt for laboratory assistance. This work was partially supported hy U.S. Public Health Service grant GM 16406