The lymph node stromal laminin α5 shapes alloimmunity

Lushen Li, Marina W. Shirkey, Tianshu Zhang, Yanbao Xiong, Wenji Piao, Vikas Saxena, Christina Paluskievicz, Young Lee, Nicholas Toney, Benjamin M. Cerel, Qinshan Li, Thomas Simon, Kyle D. Smith, Keli L. Hippen, Bruce R. Blazar, Reza Abdi, Jonathan S. Bromberg

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Lymph node stromal cells (LNSCs) regulate immunity through constructing lymphocyte niches. LNSC-produced laminin α5 (Lama5) regulates CD4+ T cells but the underlying mechanisms of its functions are poorly understood. Here we show that depleting Lama5 in LNSCs resulted in decreased Lama5 protein in the LN cortical ridge (CR) and around high endothelial venules (HEVs). Lama5 depletion affected LN structure with increased HEVs, upregulated chemokines, and cell adhesion molecules, and led to greater numbers of Tregs in the T cell zone. Mouse and human T cell transendothelial migration and T cell entry into LNs were suppressed by Lama5 through the receptors α6 integrin and α-dystroglycan. During immune responses and allograft transplantation, depleting Lama5 promoted antigen-specific CD4+ T cell entry into the CR through HEVs, suppressed T cell activation, and altered T cell differentiation to suppressive regulatory phenotypes. Enhanced allograft acceptance resulted from depleting Lama5 or blockade of T cell Lama5 receptors. Lama5 and Lama4/Lama5 ratios in allografts were associated with the rejection severity. Overall, our results demonstrated that stromal Lama5 regulated immune responses through altering LN structures and T cell behaviors. This study delineated a stromal Lama5-T cell receptor axis that can be targeted for immune tolerance modulation.

Original languageEnglish (US)
Pages (from-to)2602-2619
Number of pages18
JournalJournal of Clinical Investigation
Volume130
Issue number5
DOIs
StatePublished - May 1 2020

Bibliographical note

Funding Information:
This study was supported by NIH grants 1R01AI114496 and R01AI062765 (to JSB), R6029-07 (to BRB), R01 HL11879, P01 AI056299 (to BRB), and NCI P01 CA067493 (to BRB). We thank Ralf Adams and Lijun Xia for providing Pdgfrb-Cre mice, and Jeffrey H. Miner for supplying Lama5-floxed (CLA5fl/fl) mice. We appreciate Katrina M. Williams and Joseph P. Stains for their help with cell flow assays. We appreciate the flow cytometry sorting work performed by the University of Maryland Marlene and Stewart Greenebaum Cancer Center Flow Cytometry Shared Service.

Funding Information:
This study was supported by NIH grants 1R01AI114496 and R01AI062765 (to JSB), R6029–07 (to BRB), R01 HL11879, P01 AI056299 (to BRB), and NCI P01 CA067493 (to BRB). We thank Ralf Adams and Lijun Xia for providing Pdgfrb-Cre mice, and Jeffrey H. Miner for supplying Lama5-floxed (CLA5fl/fl) mice. We appreciate Katrina M. Williams and Joseph P. Stains for their help with cell flow assays. We appreciate the flow cytometry sorting work performed by the University of Maryland Marlene and Stewart Greenebaum Cancer Center Flow Cytometry Shared Service.

Publisher Copyright:
Copyright: © 2020, American Society for Clinical Investigation.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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