The intestinal uptake of phenol from micellar systems does not conform to the aqueous transfer model

Prajakti A. Kothare, Cheryl L. Zimmerman

Research output: Contribution to journalArticlepeer-review


Purpose. To evaluate the aqueous transfer model as the mechanism for the micelle-mediated uptake of phenol in the rat in situ intestinal perfusion model. Methods. Phenol in isotonic HEPES buffer was perfused through the jejunal segment at two flow rates and at various concentrations. Phenol was then dispersed in two, distinct mixed micelle systems composed of sodium taurocholate and phosphatidylcholine at 10 mM:2.5 mM (10:2.5 system) and at 10 mM: 10 mM (10:10 system) and its uptake studied in each case. Equilibrium dialysis was done to determine the aqueous fraction of phenol in each system. Results. The P(eff) of phenol in isotonic HEPES buffer at a low flow rate (n = 6) was 1.7 ± 0.4 x 10-4 cm/s and at a high flow rate (n = 13) was 1.8 ± 0.5 x 10-4 cm/s. The P(eff) for the 10:2.5 system at the high flow rate (n = 3) was 1.5 ± 0.4 x 10-4 cm/s and at the low flow rate (n = 3) was 1.4 ± 0.3 x 10-4 cm/s. Uptake was membrane rate-limited in both the non-micellar and 10:2.5 systems. P(eff) at a high flow rate (n = 3) in the 10:10 system was 1.3 ± 0.1 x 10-4 cm/s. Equilibrium dialysis (n = 4) revealed free fractions of 0.60 ± 0.05 and 0.50 ± 0.03 for the 10:2.5 and 10:10 systems. Conclusions. The uptake of micellized phenol did not follow the aqueous transfer model of uptake.

Original languageEnglish (US)
Pages (from-to)839-843
Number of pages5
JournalPharmaceutical research
Issue number7
StatePublished - 2000

Bibliographical note

Funding Information:
The partial financial support from ISWOP, University of Minnesota, is acknowledged. We thank Dr. Timothy S. Wied-mann for useful scientific discussions through the course of the study. The authors would also like to thank Dr. Roland Gunther, Department of Veterinary Medicine, University of Minnesota, for the analysis of the histological samples.

Copyright 2017 Elsevier B.V., All rights reserved.


  • Aqueous transfer model
  • Equilibrium dialysis
  • Micelle-mediated uptake
  • Phenol


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