The inhibitor of phagocytosis, O-phospho-l-serine, suppresses Muller glia proliferation and cone cell regeneration in the light-damaged zebrafish retina

Travis J. Bailey, Sara L. Fossum, Shane M. Fimbel, Jacob E. Montgomery, David R. Hyde

Research output: Contribution to journalArticlepeer-review

93 Scopus citations


The damaged zebrafish retina replaces lost neurons through a regenerative response that initiates with the asymmetric cell division of Müller glia to produce neuronal progenitor cells that proliferate and migrate to the damaged retinal layer, where they differentiate into the lost neuronal cell types. Because Müller glia are known to phagocytose apoptotic retinal cells during development, we tested if Müller glia engulfed apoptotic rod cell bodies in light-damaged retinas. After 24. h of constant intense light, damaged retinas revealed both a strong nuclear TUNEL signal in photoreceptors and a weak cytoplasmic TUNEL signal in Müller glia, although Müller glial apoptosis is not observed in the light-damaged retina. Light damage of a rod-specific transgenic reporter line, Tg(XlRho:EGFP)fl1, resulted in some Müller glia containing both TUNEL signal and EGFP, which indicated that this subset of Müller glia engulfed apoptotic photoreceptor cell bodies.To determine if phagocytosis induced the Müller glial proliferative response in the light-damaged retina, we utilized O-phospho-l-serine (L-SOP), a molecule that mimics the phosphatidylserine head group and partially blocks microglial phagocytosis of apoptotic cells. Intravitreal injection of L-SOP immediately prior to beginning constant intense light treatment: i) did not significantly reduce light-induced photoreceptor cell death, ii) significantly reduced the number of PCNA-positive Müller glia, and iii) significantly reduced the number of cone photoreceptors in the regenerated retina relative to control retinas. Because L-SOP is also a specific group III metabotropic glutamate receptor (mGluR) agonist, we also tested if the more potent specific group III agonist, l-2-amino-4-phosphonobutyrate (L-AP4), the specific group III antagonist (RS)-α-Methylserine-O-phosphate (MSOP) or the specific group I antagonist, l-2-amino-3-phophonopropanoic acid (L-AP3) affected Müller glial proliferation. We found no changes with any of these factors compared to control retinas, revealing that metabotropic glutamate receptors were not necessary in the Müller glia proliferative response. Furthermore, ascl1a and stat3 expression were unaffected in either the L-SOP or MSOP-injected retinas relative to controls, suggesting L-SOP disrupts Müller glia proliferation subsequent to or in parallel with ascl1a and stat3 activation. This implies that at least one signaling mechanism, in addition to the process disrupted by L-SOP, is required to activate Müller glia proliferation in the light-damaged retina.

Original languageEnglish (US)
Pages (from-to)601-612
Number of pages12
JournalExperimental Eye Research
Issue number5
StatePublished - Nov 2010

Bibliographical note

Funding Information:
We thank D. Bang and the staff of the Freimann Life Science Center for providing zebrafish husbandry and care. We thank Jonathan Scholes for the 4C4 monoclonal antibody. This work was funded by National Institutes of Health grant R01EY018417 (D.R.H.) and the Center for Zebrafish Research at the University of Notre Dame .


  • Müller glia
  • Phagocytosis
  • Phosphatidylserine
  • Regeneration
  • Retina
  • Type III metabotropic glutamate receptor


Dive into the research topics of 'The inhibitor of phagocytosis, O-phospho-l-serine, suppresses Muller glia proliferation and cone cell regeneration in the light-damaged zebrafish retina'. Together they form a unique fingerprint.

Cite this