The aim of this study was to evaluate, by confocal laser scanning microscopy (CLSM), the effect of Ca(OH)2 dressing on the depth of dentinal tubule sealer penetration and the percentage of sealer-dentin adaptation in the coronal, middle, and apical thirds of teeth filled with methacrylate- based resin sealer (Epiphany). Methods: A total of 30 extracted single-rooted human mandibular incisors were instrumented up to file #40.04 (ProFile), with 1.0% NaOCl as irrigating solution. The teeth were randomly divided into three groups (n = 10): GI = no Ca(OH)2 dressing (control group); GII = Ca(OH)2 for 14 days + removal with saline solution and #40 K-file, and GIII = similar to Group II plus 17% EDTA for dressing removal. The Epiphany sealer was labeled with Rhodamine B dye, and all canals were filled with the Epiphany system. Three sections of each tooth were sectioned and viewed under 5X and 40X magnifications. Results: ANOVA and Tukey tests showed significantly lower values of sealer penetration depth in the apical third compared to the middle and coronal thirds (p < 0.05). The deepest sealer penetration was observed in the coronal and middle thirds of Group II. Groups II (93%) and III (86%) had the highest percentage of adaptation compared with Group I (78%) (p < 0.05). Conclusions: Ca(OH)2 favors the depth of methacrylate-based sealer penetration and the percentage of sealer-dentin adaptation in teeth filled with the Epiphany system.
|Original language||English (US)|
|Number of pages||7|
|Journal||Dental Press Endodontics|
|State||Published - 2015|
Bibliographical notePublisher Copyright:
© 2015 Dental Press Endodontics.
- Calcium hydroxide
- Confocal laser scanning microscopy
- Fluorescent dye
- Root canal filling