Three experiments were conducted to evaluate the effect of immobilization on plasma prolactin (PRL) levels in the immature turkey. Acute immobilization for 120 min resulted in elevated PRL levels (P < 0.05) starting 15 min after the onset of immobilization. Release from immobilization caused PRL levels to return to those of the nonimmobilized controls by 120-180 min following replacement of the turkeys in cages. Repeated immobilization for 3 or 5 consecutive days diminished PRL response (P < 0.05) to subsequent immobilization. It is suggested that the PRL controlling mechanism(s) of the young turkey is both susceptible and able to habituate to changes induced by immobilization stress.