The impact of TCR signal strength on resident memory T cell formation during influenza virus infection

Jessica K. Fiege, Ian A. Stone, Elizabeth J. Fay, Matthew W. Markman, Sathi Wijeyesinghe, Marissa Macchietto, Steven Shen, David Masopust, Ryan A Langlois

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2 Scopus citations


Resident memory T cells (TRM) in the lung are vital for heterologous protection against influenza A virus (IAV). Environmental factors are necessary to establish lung TRM; however, the role of T cell–intrinsic factors like TCR signal strength have not been elucidated. In this study, we investigated the impact of TCR signal strength on the generation and maintenance of lung TRM after IAV infection. We inserted high- and low-affinity OT-I epitopes into IAV and infected mice after transfer of OT-I T cells. We uncovered a bias in TRM formation in the lung elicited by lower affinity TCR stimulation. TCR affinity did not impact the overall phenotype or long-term maintenance of lung TRM. Overall, these findings demonstrate that TRM formation is negatively correlated with increased TCR signal strength. Lower affinity cells may have an advantage in forming TRM to ensure diversity in the Ag-specific repertoire in tissues.

Original languageEnglish (US)
Pages (from-to)936-945
Number of pages10
JournalJournal of Immunology
Issue number4
StatePublished - Aug 15 2019


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