The impact of HER2 overexpression on the miRNA and circRNA transcriptomes in two breast cell lines and their vesicles

Maria Galli De Amorim, Gabriela Branco, Renan Valieris, Emilio Tarcitano, Israel Tojal Da Silva, Luiza Ferreira De Araújo, Diana Noronha Nunes, Emmanuel Dias-Neto

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

HER2 upregulation is related with poor outcome in many tumor types. Whereas anti-HER2 treatment is the standard approach as adjuvant therapy in HER2-overexpressing breast cancer, the frequent relapses reinforce the need for alternative treatments. Here we used next-generation sequencing (NGS) to evaluate miRNAs and circRNAs in the cell-lines HB4a and C5.2, where the latter is a HER2-overexpressing clone of the former, and also from two different populations of their secreted extracellular vesicles. Whereas circRNA-levels were stable, we found at least 16 miRNAs apparently modulated by HER2-expression. The miR223-3p, miR-421 and miR-21-5p were validated in an independent cohort of 431 breast cancer patients from The Cancer Genome Atlas (TCGA). The consistent modulation of these molecules and their possible involvement in the HER2-axis makes them promising new targets to overcome HER2-activation.

Original languageEnglish (US)
Pages (from-to)493-502
Number of pages10
JournalPharmacogenomics
Volume20
Issue number7
DOIs
StatePublished - May 24 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 Future Medicine Ltd.

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