The impact of discrete modes of spinal cord injury on bladder muscle contractility

Abhishek Seth; Yeun Goo Chung; Daniel Kim; Aruna Ramachandran; Vivian Cristofaro; Pablo Gomez; Duong Tu; Lin Huang; Larry I. Benowitz; Dolores Di Vizio; Maryrose P. Sullivan; Rosalyn M. Adam

doi:10.1186/1471-2490-13-24

The impact of discrete modes of spinal cord injury on bladder muscle contractility

Abhishek Seth, Yeun Goo Chung, Daniel Kim, Aruna Ramachandran, Vivian Cristofaro, Pablo Gomez, Duong Tu, Lin Huang, Larry I. Benowitz, Dolores Di Vizio, Maryrose P. Sullivan, Rosalyn M. Adam

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Background: Prior studies have compared the effect of spinal cord injury elicited using distinct approaches on motor and visceral function. However, the impact of such discrete modes of injury specifically on bladder muscle contractility has not been explored in detail. The goal of this study is to compare the impact of complete spinal cord transection versus clip compression at thoracic vertebra eight (T8) on bladder muscle contractility. Methods. Rats underwent no treatment (Control), laminectomy (Sham, SH); complete extradural transection (TX); or cord compression with an aneurysm clip (CX). Bladders and spinal cords were harvested at 6 wk for contractility studies or histological analysis. Results: Detrusor strips from TX and CX rats showed higher spontaneous activity than those from SH rats. Furthermore, the duration of the neurally-mediated contractile response was longer in TX and CX rats compared to controls and showed attenuated relaxation. No significant differences were observed between muscle strips from SH, TX or CX rats in response to KCl, ATP or phenylephrine. However, tissues from TX and CX rats showed a higher sensitivity to carbachol compared to that from SH animals. Conclusions: Complete SCI in rats either by cord transection or compression elicits qualitatively similar changes in bladder muscle contractility. Whereas cord transection is arguably easier to perform experimentally, cord compression better models the situation observed clinically, such that each approach has clear advantages and limitations.

Original language	English (US)
Article number	24
Journal	BMC Urology
Volume	13
DOIs	https://doi.org/10.1186/1471-2490-13-24
State	Published - 2013
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank members of the Urological Diseases Research Center at Boston Children’s Hospital for helpful discussions, as well as Dr. Peter Zvara and colleagues at the University of Vermont for their help in optimizing functional analysis in rodents. The authors acknowledge financial support from the Children’s Urological Foundation and from the National Institutes of Health (R01 DK077195 and P50 DK65298 to R.M.A.; R00 CA131472 to D.D.V.) and the Medical Research Service, Department of Veterans Affairs (M.P.S).

Keywords

Bladder
Muscle contractility
Smooth muscle
Spinal cord injury

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10.1186/1471-2490-13-24

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title = "The impact of discrete modes of spinal cord injury on bladder muscle contractility",

abstract = "Background: Prior studies have compared the effect of spinal cord injury elicited using distinct approaches on motor and visceral function. However, the impact of such discrete modes of injury specifically on bladder muscle contractility has not been explored in detail. The goal of this study is to compare the impact of complete spinal cord transection versus clip compression at thoracic vertebra eight (T8) on bladder muscle contractility. Methods. Rats underwent no treatment (Control), laminectomy (Sham, SH); complete extradural transection (TX); or cord compression with an aneurysm clip (CX). Bladders and spinal cords were harvested at 6 wk for contractility studies or histological analysis. Results: Detrusor strips from TX and CX rats showed higher spontaneous activity than those from SH rats. Furthermore, the duration of the neurally-mediated contractile response was longer in TX and CX rats compared to controls and showed attenuated relaxation. No significant differences were observed between muscle strips from SH, TX or CX rats in response to KCl, ATP or phenylephrine. However, tissues from TX and CX rats showed a higher sensitivity to carbachol compared to that from SH animals. Conclusions: Complete SCI in rats either by cord transection or compression elicits qualitatively similar changes in bladder muscle contractility. Whereas cord transection is arguably easier to perform experimentally, cord compression better models the situation observed clinically, such that each approach has clear advantages and limitations.",

keywords = "Bladder, Muscle contractility, Smooth muscle, Spinal cord injury",

author = "Abhishek Seth and Chung, {Yeun Goo} and Daniel Kim and Aruna Ramachandran and Vivian Cristofaro and Pablo Gomez and Duong Tu and Lin Huang and Benowitz, {Larry I.} and {Di Vizio}, Dolores and Sullivan, {Maryrose P.} and Adam, {Rosalyn M.}",

note = "Funding Information: The authors would like to thank members of the Urological Diseases Research Center at Boston Children{\textquoteright}s Hospital for helpful discussions, as well as Dr. Peter Zvara and colleagues at the University of Vermont for their help in optimizing functional analysis in rodents. The authors acknowledge financial support from the Children{\textquoteright}s Urological Foundation and from the National Institutes of Health (R01 DK077195 and P50 DK65298 to R.M.A.; R00 CA131472 to D.D.V.) and the Medical Research Service, Department of Veterans Affairs (M.P.S).",

year = "2013",

doi = "10.1186/1471-2490-13-24",

language = "English (US)",

volume = "13",

journal = "BMC Urology",

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TY - JOUR

T1 - The impact of discrete modes of spinal cord injury on bladder muscle contractility

AU - Seth, Abhishek

AU - Chung, Yeun Goo

AU - Kim, Daniel

AU - Ramachandran, Aruna

AU - Cristofaro, Vivian

AU - Gomez, Pablo

AU - Tu, Duong

AU - Huang, Lin

AU - Benowitz, Larry I.

AU - Di Vizio, Dolores

AU - Sullivan, Maryrose P.

AU - Adam, Rosalyn M.

N1 - Funding Information: The authors would like to thank members of the Urological Diseases Research Center at Boston Children’s Hospital for helpful discussions, as well as Dr. Peter Zvara and colleagues at the University of Vermont for their help in optimizing functional analysis in rodents. The authors acknowledge financial support from the Children’s Urological Foundation and from the National Institutes of Health (R01 DK077195 and P50 DK65298 to R.M.A.; R00 CA131472 to D.D.V.) and the Medical Research Service, Department of Veterans Affairs (M.P.S).

PY - 2013

Y1 - 2013

N2 - Background: Prior studies have compared the effect of spinal cord injury elicited using distinct approaches on motor and visceral function. However, the impact of such discrete modes of injury specifically on bladder muscle contractility has not been explored in detail. The goal of this study is to compare the impact of complete spinal cord transection versus clip compression at thoracic vertebra eight (T8) on bladder muscle contractility. Methods. Rats underwent no treatment (Control), laminectomy (Sham, SH); complete extradural transection (TX); or cord compression with an aneurysm clip (CX). Bladders and spinal cords were harvested at 6 wk for contractility studies or histological analysis. Results: Detrusor strips from TX and CX rats showed higher spontaneous activity than those from SH rats. Furthermore, the duration of the neurally-mediated contractile response was longer in TX and CX rats compared to controls and showed attenuated relaxation. No significant differences were observed between muscle strips from SH, TX or CX rats in response to KCl, ATP or phenylephrine. However, tissues from TX and CX rats showed a higher sensitivity to carbachol compared to that from SH animals. Conclusions: Complete SCI in rats either by cord transection or compression elicits qualitatively similar changes in bladder muscle contractility. Whereas cord transection is arguably easier to perform experimentally, cord compression better models the situation observed clinically, such that each approach has clear advantages and limitations.

AB - Background: Prior studies have compared the effect of spinal cord injury elicited using distinct approaches on motor and visceral function. However, the impact of such discrete modes of injury specifically on bladder muscle contractility has not been explored in detail. The goal of this study is to compare the impact of complete spinal cord transection versus clip compression at thoracic vertebra eight (T8) on bladder muscle contractility. Methods. Rats underwent no treatment (Control), laminectomy (Sham, SH); complete extradural transection (TX); or cord compression with an aneurysm clip (CX). Bladders and spinal cords were harvested at 6 wk for contractility studies or histological analysis. Results: Detrusor strips from TX and CX rats showed higher spontaneous activity than those from SH rats. Furthermore, the duration of the neurally-mediated contractile response was longer in TX and CX rats compared to controls and showed attenuated relaxation. No significant differences were observed between muscle strips from SH, TX or CX rats in response to KCl, ATP or phenylephrine. However, tissues from TX and CX rats showed a higher sensitivity to carbachol compared to that from SH animals. Conclusions: Complete SCI in rats either by cord transection or compression elicits qualitatively similar changes in bladder muscle contractility. Whereas cord transection is arguably easier to perform experimentally, cord compression better models the situation observed clinically, such that each approach has clear advantages and limitations.

KW - Bladder

KW - Muscle contractility

KW - Smooth muscle

KW - Spinal cord injury

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DO - 10.1186/1471-2490-13-24

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AN - SCOPUS:84877628966

SN - 1471-2490

VL - 13

JO - BMC Urology

JF - BMC Urology

M1 - 24

ER -

The impact of discrete modes of spinal cord injury on bladder muscle contractility

Abstract

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