The identification and characterization of a testisspecific cDNA during spermatogenesis

Ying Chen, Jiarui Hu, Ping Song, Wuming Gong

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Using bioinformatics and experimental validation, we obtained a cDNA (named srsf) which was exclusively expressed in the mouse testes. RTPCR analysis showed that srsf mRNA was not expressed in the gonad during the sex determination period or during embryogenesis. In developing mouse testes, srsf expression was first detected on post-natal day 10, reached its highest level on day 23, and then reduced to and remained at a moderate level throughout adulthood. In situ hybridization analysis demonstrated that srsf mRNA was expressed in pachytene spermatocytes and round spermatids in the testes. The predicted protein contains one RNA-binding domain (RBD) and a serine-arginine rich domain (RS), which are characterized by some splicing factors of SR family members. These findings indicate that srsf may play a role during spermatogenesis.

Original languageEnglish (US)
Pages (from-to)80-89
Number of pages10
JournalCellular and Molecular Biology Letters
Volume11
Issue number1
DOIs
StatePublished - 2006

Bibliographical note

Funding Information:
Acknowledgements. This study was supported by grants from the ICGEB (International Centre for Genetic Engineering and Biotechnology) (CRP/CHN02-01), National Nature Science Foundation of China (No. 30270675) and the National Basic Research Program of China (2004CB117400).

Keywords

  • Expression pattern
  • Spermatogenesis
  • Splicing factor

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