The Human ApoE4 Variant Reduces Functional Recovery and Neuronal Sprouting After Incomplete Spinal Cord Injury in Male Mice

Carlos A. Toro, Jens Hansen, Mustafa M. Siddiq, Kaitlin Johnson, Wei Zhao, Daniella Azulai, Dibash K. Das, William Bauman, Robert Sebra, Dongming Cai, Ravi Iyengar, Christopher P. Cardozo

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Spinal cord injury (SCI) is a devastating form of neurotrauma. Patients who carry one or two apolipoprotein E (ApoE)4 alleles show worse functional outcomes and longer hospital stays after SCI, but the cellular and molecular underpinnings for this genetic link remain poorly understood. Thus, there is a great need to generate animal models to accurately replicate the genetic determinants of outcomes after SCI to spur development of treatments that improve physical function. Here, we examined outcomes after a moderate contusion SCI of transgenic mice expressing human ApoE3 or ApoE4. ApoE4 mice have worse locomotor function and coordination after SCI. Histological examination revealed greater glial staining in ApoE4 mice after SCI associated with reduced levels of neuronal sprouting markers. Bulk RNA sequencing revealed that subcellular processes (SCPs), such as extracellular matrix organization and inflammatory responses, were highly ranked among upregulated genes at 7 days after SCI in ApoE4 variants. Conversely, SCPs related to neuronal action potential and neuron projection development were increased in ApoE3 mice at 21 days. In summary, our results reveal a clinically relevant SCI mouse model that recapitulates the influence of ApoE genotypes on post SCI function in individuals who carry these alleles and suggest that the mechanisms underlying worse recovery for ApoE4 animals involve glial activation and loss of sprouting and synaptic activity.

Original languageEnglish (US)
Article number626192
JournalFrontiers in Cellular Neuroscience
StatePublished - Feb 18 2021
Externally publishedYes

Bibliographical note

Funding Information:
We are grateful to Dr. Miguel Gama-Sosa for many helpful discussions about technical aspects of the studies reported herein. Funding. This work was supported by the New York State, Department of Health, Wadsworth Center, Innovative, Developmental or Exploratory Activities (IDEA) in Spinal Cord (NYS SCIRB Grant# DOH01-PART2-2017-00035 to CC and DC), the Department of Veterans Affairs Rehabilitation and Development Service Grant B-2020-C, and the James J. Peters VA Medical Center. JH, MS, and RI were supported by GM54508 and now by GM137056.

Publisher Copyright:
© Copyright © 2021 Toro, Hansen, Siddiq, Johnson, Zhao, Azulai, Das, Bauman, Sebra, Cai, Iyengar and Cardozo.


  • ApoE
  • functional recovery after SCI
  • glial activation
  • neurite outgrowth
  • RNA-seq transcriptomics
  • spinal cord injury
  • sprouting


Dive into the research topics of 'The Human ApoE4 Variant Reduces Functional Recovery and Neuronal Sprouting After Incomplete Spinal Cord Injury in Male Mice'. Together they form a unique fingerprint.

Cite this