The genome of the truffle-parasite Tolypocladium ophioglossoides and the evolution of antifungal peptaibiotics

C. Alisha Quandt, Kathryn E. Bushley, Joseph W. Spatafora

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36 Scopus citations


Background: Two major mycoparasitic lineages, the family Hypocreaceae and the genus Tolypocladium, exist within the fungal order, Hypocreales. Peptaibiotics are a group of secondary metabolites almost exclusively described from Trichoderma species of Hypocreaceae. Peptaibiotics are produced by nonribosomal peptide synthetases (NRPSs) and have antibiotic and antifungal activities. Tolypocladium species are mainly truffle parasites, but a few species are insect pathogens. Results: The draft genome sequence of the truffle parasite Tolypocladium ophioglossoides was generated and numerous secondary metabolite clusters were discovered, many of which have no known putative product. However, three large peptaibiotic gene clusters were identified using phylogenetic analyses. Peptaibiotic genes are absent from the predominantly plant and insect pathogenic lineages of Hypocreales, and are therefore exclusive to the largely mycoparasitic lineages. Using NRPS adenylation domain phylogenies and reconciliation of the domain tree with the organismal phylogeny, it is demonstrated that the distribution of these domains is likely not the product of horizontal gene transfer between mycoparasitic lineages, but represents independent losses in insect pathogenic lineages. Peptaibiotic genes are less conserved between species of Tolypocladium and are the product of complex patterns of lineage sorting and module duplication. In contrast, these genes are more conserved within the genus Trichoderma and consistent with diversification through speciation. Conclusions: Peptaibiotic NRPS genes are restricted to mycoparasitic lineages of Hypocreales, based on current sampling. Phylogenomics and comparative genomics can provide insights into the evolution of secondary metabolite genes, their distribution across a broader range of taxa, and their possible function related to host specificity.

Original languageEnglish (US)
Article number553
JournalBMC Genomics
Issue number1
StatePublished - Jul 28 2015

Bibliographical note

Funding Information:
Fig. 3 Phylogeny of the three peptaibol A-domain clades. Peptaibol focused, A-domain phylogeny created using RAxML, showing the 3 major clades. Green branches represent those supported by ≥70 % bootstrap support. Tolypocladium spp. A-domains are colored in blue and teal, and Trichoderma spp. A-domains are colored in yellow, red, or orange

Publisher Copyright:
© 2015 Quandt et al.


  • Hypocreales
  • Lineage sorting
  • Mycoparasites
  • Secondary metabolism


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