CTP:phosphocholine cytidylyltransferase is the rate-controlling enzyme in phosphatidylcholine biosynthesis and is essential for the survival of eukaryotic cells. The murine cDNA for the cytidylyltransferase was cloned and sequenced. A genomic clone was isolated and the chromosomal location of the Ctpct locus determined by Southern blot hybridization of DNAs from a panel of interspecific backcross progeny derived from matings of [(C57BL/6J × Mus spretus)F1 × C57BL/6J] mice. These data place the Ctpct gene on mouse chromosome 16 between the Smst and Stf-1 genes.
Bibliographical noteFunding Information:
We thank M. Barnstead for excellent technical assistance. This work was supported by National Institutes of Health Grant GM45737 from the National Institute of General Medical Sciences, National Research Service Award T32 CA09346 from the National Cancer Institute (to T.G.T.), Cancer Center (CORE) Grant CA21765 from the National Cancer Institute, the American Lebanese Syrian Associated Charities, and the National Cancer Institute, DHHS, under Contract NO1-CO-74101 with ABL.