γ-Aminobutyric acid (GABA)(A) receptors are the sites of action for many antiepileptic drugs such as benzodiazepines and barbiturates. We report the results of molecular cloning of the γ1-subunit from seizure prone DBA/2J and resistant C57BL/6J inbred mice, and analyses of nucleotide sequences and expression of the γ1-subunit messenger RNA (mRNA) in DBA/2 and C57BL/6 inbred mice. The mouse γ1-subunit complementary DNA (cDNA) shares 98% similarity with that of the rat at the level of amino acid sequence. Northern blot hybridization indicates that the γ1-subunit mRNA is expressed predominantly in areas other than the cerebral cortex and cerebellum and shows little change with postnatal development. No differences have been found for the subunit between DBA/2 and C57BL/6 mice either for nucleotide sequence or for level of expression of the subunit's mRNA in whole brain by Northern blots at 3 weeks of age.
Bibliographical noteFunding Information:
This work was supported in part by USPHS grants HD16596, NS25525, and AAO7559. The authors thank David Overman for assistance in cDNA library screening and Dr. S.J. Moss for some PCR primers and advice. Some of the work in this paper was in partial fulfillment of the requirements for the Ph.D. degree of J.B. Wang at the University of Maryland School of Medicine.
- GABA/benzodiazepine receptor