The function of heterodimeric AP-1 comprised of C-jun and C-fos in activin mediated spemann organizer gene expression

Sung Young Lee, Jaeho Yoon, Hyun Shik Lee, Yoo Seok Hwang, Sang Wook Cha, Chul Ho Jeong, Jong Il Kim, Jae Bong Park, Jae Yong Lee, Sung Chan Kim, Mae Ja Park, Zigang Dong, Jaebong Kim

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Background: Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development. Methodology/Principal Findings: We provide new evidence showing that overexpression of heterodimeric AP-1 comprised of c-jun and c-fos (AP-1 c-Jun/c-Fos) induces the expression of BMP-antagonizing organizer genes (noggin, chordin and goosecoid) that were normally expressed by high dose of activin. AP-1 c-Jun/c-Fos enhanced the promoter activities of organizer genes but reduced that of PV.1, a BMP4-response gene. A loss of function study clearly demonstrated that AP-1 c-Jun/c-Fos is required for the activin-induced organizer and neural gene expression. Moreover, physical interaction of AP-1 c-Jun/c-Fos and Smad3 cooperatively enhanced the transcriptional activity of goosecoid via direct binding on this promoter. Interestingly, Smad3 mutants at c-Jun binding site failed in regulation of organizer genes, indicating that these physical interactions are specifically necessary for the expression of organizer genes. Conclusions/Significance: AP-1 c-Jun/c-Fos plays a specific role in organizer gene expression in downstream of activin signal during early Xenopus embryogenesis.

Original languageEnglish (US)
Article numbere21796
JournalPloS one
Volume6
Issue number7
DOIs
StatePublished - 2011

Fingerprint

Dive into the research topics of 'The function of heterodimeric AP-1 comprised of C-jun and C-fos in activin mediated spemann organizer gene expression'. Together they form a unique fingerprint.

Cite this