The function of a Drosophila glypican does not depend entirely on heparan sulfate modification

Catherine Kirkpatrick, Sarah M. Knox, William D. Staatz, Bethany Fox, Daniel M. Lercher, Scott B. Selleck

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Division abnormally delayed (Dally) is one of two glycosylphosphatidylinositol (GPI)-linked heparan sulfate proteoglycans in Drosophila. Numerous studies have shown that it influences Decapentaplegic (Dpp) and Wingless signaling. It has been generally assumed that Dally affects signaling by directly interacting with these growth factors, primarily through its heparan sulfate (HS) chains. To understand the functional contributions of HS chains and protein core we have (1) assessed the growth factor binding properties of purified Dally using surface plasmon resonance, (2) generated a form of Dally that is not HS modified and evaluated its signaling capacity in vivo. Purified Dally binds directly to FGF2, FGF10, and the functional Dpp homolog BMP4. FGF binding is abolished by preincubation with HS, but BMP4 association is partially HS-resistant, suggesting the Dally protein core contributes to binding. Cell binding and co-immunoprecipitation studies suggest that non-HS-modified Dally retains some ability to bind Dpp or BMP4. Expression of HS-deficient Dally in vivo showed it does not promote signaling as well as wild-type Dally, yet it can rescue several dally mutant phenotypes. These data reveal that heparan sulfate modification of Dally is not required for all in vivo activities and that significant functional capacity resides in the protein core.

Original languageEnglish (US)
Pages (from-to)570-582
Number of pages13
JournalDevelopmental Biology
Volume300
Issue number2
DOIs
StatePublished - Dec 15 2006

Fingerprint

Glypicans
Heparitin Sulfate
Drosophila
Intercellular Signaling Peptides and Proteins
Heparan Sulfate Proteoglycans
Glycosylphosphatidylinositols
Proteins
Surface Plasmon Resonance
Fibroblast Growth Factor 2
Immunoprecipitation
Sulfates
Phenotype

Keywords

  • BMP
  • Dally
  • Decapentaplegic
  • Drosophila
  • FGF
  • Glypican
  • Heparan sulfate
  • Proteoglycan
  • Wingless

Cite this

The function of a Drosophila glypican does not depend entirely on heparan sulfate modification. / Kirkpatrick, Catherine; Knox, Sarah M.; Staatz, William D.; Fox, Bethany; Lercher, Daniel M.; Selleck, Scott B.

In: Developmental Biology, Vol. 300, No. 2, 15.12.2006, p. 570-582.

Research output: Contribution to journalArticle

Kirkpatrick, Catherine ; Knox, Sarah M. ; Staatz, William D. ; Fox, Bethany ; Lercher, Daniel M. ; Selleck, Scott B. / The function of a Drosophila glypican does not depend entirely on heparan sulfate modification. In: Developmental Biology. 2006 ; Vol. 300, No. 2. pp. 570-582.
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