Products of Ig κ L chain gene rearrangement in a variety of human B cell samples were investigated by sequential Southern blot hybridization analysis. By application of four region-specific probes (C(κ), J(κ), U'(κ) and κde) a complete spectrum of κ rearrangements, including both predicted and novel products, were detected. Nearly 30% of the products detected reflect multiple recombination of the κ locus. The κ-deleting element was responsible for 70% of the multiple rearrangements that were detected. Interestingly, eight κ-expressing samples exhibited rearrangement of the κ-deleting element. The remaining multiple recombination products were characteristic of double V(κ)-J(κ) rearrangement. This frequency reveals that secondary V-J rearrangement may significantly contribute to the expression of κ L chains in humans.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Immunology|
|State||Published - 1990|