The frequency of multiple recombination events occurring at the human Ig κ L chain locus

Rodney M. Feddersen, Debra J. Martin, Brian G. Van Ness

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Products of Ig κ L chain gene rearrangement in a variety of human B cell samples were investigated by sequential Southern blot hybridization analysis. By application of four region-specific probes (Cκ, Jκ, U′κ and κde) a complete spectrum of κ rearrangements, including both predicted and novel products, were detected. Nearly 30% of the products detected reflect multiple recombination of the κ locus. The κ-deleting element was responsible for 70% of the multiple rearrangements that were detected. Interestingly, eight κ-expressing samples exhibited rearrangement of the κ-deleting element. The remaining multiple recombination products were characteristic of double Vκ-Jκ rearrangement. This frequency reveals that secondary V-J rearrangement may significantly contribute to the expression of κ L chains in humans.

Original languageEnglish (US)
Pages (from-to)1088-1093
Number of pages6
JournalJournal of Immunology
Volume144
Issue number3
StatePublished - Feb 1 1990

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