The four-and-a-half-LIM protein 2 (FHL2) is overexpressed in gliomas and associated with oncogenic activities

Ming Li, Jide Wang, Samuel S.M. Ng, Chu Yan Chan, Amy C. Chen, Hong Ping Xia, David T. Yew, Benjamin C.Y. Wong, Zhu Chen, Hsiang Fu Kung, Marie Chia Mi Li

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Four-and-a-half-LIM protein 2 (FHL2) is a member of FHL protein family, which plays a crucial role in regulating gene expression, cell survival, and migration. Although its function in oncogenesis appears to be tumor type-specific, its roles in glioma formation and development are yet to be elucidated. In the present study, we demonstrated that the mRNA level of FHL2 was elevated in both low- and high-grade glioma samples. Overexpression of FHL2 stimulated the proliferation, anchorage-independent growth, and migration of human glioblastoma cells. Conversely, FHL2 knockdown by short hairpin RNA (shRNA-FHL2) inhibited glioblastoma cell proliferation and migration. Overexpression of FHL2 increased the tumorigenicity of glioblastoma cells in nude mice and decreased the mRNA levels of p53 and its downstream proapoptotic genes, including p21, Bcl2-associated protein X (Bax), and p53-upregulated modulator of apoptosis. It also enhanced the promoter activities of activator protein-1 (AP-1), human telomerase reverse transcriptase, and survivin genes. Together, these results provide the first evidence that FHL2 contributes to glioma carcinogenesis.

Original languageEnglish (US)
Pages (from-to)1328-1338
Number of pages11
JournalGlia
Volume56
Issue number12
DOIs
StatePublished - Sep 1 2008
Externally publishedYes

Keywords

  • Cell migration
  • Cell proliferation
  • FHL2
  • Glioma
  • Tumorigenesis

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