The fibrotic niche impairs satellite cell function and muscle regeneration in mouse models of Marfan syndrome

Meiricris Tomaz da Silva; Audrei R. Santos; Tatiana E. Koike; Tabata L. Nascimento; Andrei Rozanski; Darko Bosnakovski; Lygia V. Pereira; Ashok Kumar; Michael Kyba; Elen H. Miyabara

doi:10.1111/apha.13889

The fibrotic niche impairs satellite cell function and muscle regeneration in mouse models of Marfan syndrome

Meiricris Tomaz da Silva, Audrei R. Santos, Tatiana E. Koike, Tabata L. Nascimento, Andrei Rozanski, Darko Bosnakovski, Lygia V. Pereira, Ashok Kumar, Michael Kyba, Elen H. Miyabara

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Aim: It has been suggested that the proliferation and early differentiation of myoblasts are impaired in Marfan syndrome (MFS) mice during muscle regeneration. However, the underlying cellular and molecular mechanisms remain poorly understood. Here, we investigated muscle regeneration in MFS mouse models by analyzing the influence of the fibrotic niche on satellite cell function. Methods: In vivo, ex vivo, and in vitro experiments were performed. In addition, we evaluated the effect of the pharmacological inhibition of fibrosis using Ang-(1–7) on regenerating skeletal muscles of MFS mice. Results: The skeletal muscle of MFS mice shows an increased accumulation of collagen fibers (81.2%), number of fibroblasts (157.1%), and Smad2/3 signaling (110.5%), as well as an aberrant number of fibro-adipogenic progenitor cells in response to injury compared with wild-type mice. There was an increased number of proinflammatory and anti-inflammatory macrophages (3.6- and 3.1-fold, respectively) in regenerating muscles of wild-type mice, but not in the regenerating muscles of MFS mice. Our data show that proliferation and differentiation of satellite cells are altered (p ≤ 0.05) in MFS mice. Myoblast transplantation assay revealed that the regenerating muscles from MFS mice have reduced satellite cell self-renewal capacity (74.7%). In addition, we found that treatment with Ang-(1–7) reduces fibrosis (71.6%) and ameliorates satellite cell dysfunction (p ≤ 0.05) and muscle contractile function (p ≤ 0.05) in MFS mice. Conclusion: The fibrotic niche, caused by Fbn1 mutations, reduces the myogenic potential of satellite cells, affecting structural and functional muscle regeneration. In addition, the fibrosis inhibitor Ang-(1–7) partially counteracts satellite cell abnormalities and restores myofiber size and contractile force in regenerating muscles.

Original language	English (US)
Article number	e13889
Journal	Acta Physiologica
Volume	237
Issue number	1
DOIs	https://doi.org/10.1111/apha.13889
State	Published - Jan 2023

Bibliographical note

Funding Information:
This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Grant Nos. 14/23391–8, 18/24946–4, 20/15351–7) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Fellowship/Grant No. 314857/2021–4) to E.H.M. M.T.S. received post‐doctoral fellowships from FAPESP and FAPESP/CAPES (Grant Nos. 17/14115–5 and 14/13874–1, respectively). A.R.S., T.E.K., and T.L.N. received Ph.D. fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES to A.R.S.) and FAPESP (Grant Nos. 17/09069–4 and 13/04783–0 to T.E.K. and T.L.N., respectively).

Funding Information:
We thank Gabriel U. Sato for technical assistance and André Cruz and Anselmo S. Moriscot for help with bioinformatics. The monoclonal antibody to MyHC was obtained from the Developmental Studies. Hybridoma Bank was developed under the sponsorships of the NICHD and maintained by the University of Iowa.

Publisher Copyright:
© 2022 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.

Keywords

Marfan syndrome
angiotensin-1-7
fibrosis
satellite cell function
skeletal muscle regeneration

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1111/apha.13889

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@article{988bafcbb82f4056b14b066bf986e7ab,

title = "The fibrotic niche impairs satellite cell function and muscle regeneration in mouse models of Marfan syndrome",

abstract = "Aim: It has been suggested that the proliferation and early differentiation of myoblasts are impaired in Marfan syndrome (MFS) mice during muscle regeneration. However, the underlying cellular and molecular mechanisms remain poorly understood. Here, we investigated muscle regeneration in MFS mouse models by analyzing the influence of the fibrotic niche on satellite cell function. Methods: In vivo, ex vivo, and in vitro experiments were performed. In addition, we evaluated the effect of the pharmacological inhibition of fibrosis using Ang-(1–7) on regenerating skeletal muscles of MFS mice. Results: The skeletal muscle of MFS mice shows an increased accumulation of collagen fibers (81.2%), number of fibroblasts (157.1%), and Smad2/3 signaling (110.5%), as well as an aberrant number of fibro-adipogenic progenitor cells in response to injury compared with wild-type mice. There was an increased number of proinflammatory and anti-inflammatory macrophages (3.6- and 3.1-fold, respectively) in regenerating muscles of wild-type mice, but not in the regenerating muscles of MFS mice. Our data show that proliferation and differentiation of satellite cells are altered (p ≤ 0.05) in MFS mice. Myoblast transplantation assay revealed that the regenerating muscles from MFS mice have reduced satellite cell self-renewal capacity (74.7%). In addition, we found that treatment with Ang-(1–7) reduces fibrosis (71.6%) and ameliorates satellite cell dysfunction (p ≤ 0.05) and muscle contractile function (p ≤ 0.05) in MFS mice. Conclusion: The fibrotic niche, caused by Fbn1 mutations, reduces the myogenic potential of satellite cells, affecting structural and functional muscle regeneration. In addition, the fibrosis inhibitor Ang-(1–7) partially counteracts satellite cell abnormalities and restores myofiber size and contractile force in regenerating muscles.",

keywords = "Marfan syndrome, angiotensin-1-7, fibrosis, satellite cell function, skeletal muscle regeneration",

author = "{Tomaz da Silva}, Meiricris and Santos, {Audrei R.} and Koike, {Tatiana E.} and Nascimento, {Tabata L.} and Andrei Rozanski and Darko Bosnakovski and Pereira, {Lygia V.} and Ashok Kumar and Michael Kyba and Miyabara, {Elen H.}",

note = "Funding Information: This work was supported by grants from Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo (FAPESP) (Grant Nos. 14/23391–8, 18/24946–4, 20/15351–7) and Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) (Fellowship/Grant No. 314857/2021–4) to E.H.M. M.T.S. received post‐doctoral fellowships from FAPESP and FAPESP/CAPES (Grant Nos. 17/14115–5 and 14/13874–1, respectively). A.R.S., T.E.K., and T.L.N. received Ph.D. fellowships from Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior (CAPES to A.R.S.) and FAPESP (Grant Nos. 17/09069–4 and 13/04783–0 to T.E.K. and T.L.N., respectively). Funding Information: We thank Gabriel U. Sato for technical assistance and Andr{\'e} Cruz and Anselmo S. Moriscot for help with bioinformatics. The monoclonal antibody to MyHC was obtained from the Developmental Studies. Hybridoma Bank was developed under the sponsorships of the NICHD and maintained by the University of Iowa. Publisher Copyright: {\textcopyright} 2022 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.",

year = "2023",

month = jan,

doi = "10.1111/apha.13889",

language = "English (US)",

volume = "237",

journal = "Acta Physiologica",

issn = "1748-1708",

publisher = "John Wiley and Sons Ltd",

number = "1",

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TY - JOUR

T1 - The fibrotic niche impairs satellite cell function and muscle regeneration in mouse models of Marfan syndrome

AU - Tomaz da Silva, Meiricris

AU - Santos, Audrei R.

AU - Koike, Tatiana E.

AU - Nascimento, Tabata L.

AU - Rozanski, Andrei

AU - Bosnakovski, Darko

AU - Pereira, Lygia V.

AU - Kumar, Ashok

AU - Kyba, Michael

AU - Miyabara, Elen H.

N1 - Funding Information: This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Grant Nos. 14/23391–8, 18/24946–4, 20/15351–7) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Fellowship/Grant No. 314857/2021–4) to E.H.M. M.T.S. received post‐doctoral fellowships from FAPESP and FAPESP/CAPES (Grant Nos. 17/14115–5 and 14/13874–1, respectively). A.R.S., T.E.K., and T.L.N. received Ph.D. fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES to A.R.S.) and FAPESP (Grant Nos. 17/09069–4 and 13/04783–0 to T.E.K. and T.L.N., respectively). Funding Information: We thank Gabriel U. Sato for technical assistance and André Cruz and Anselmo S. Moriscot for help with bioinformatics. The monoclonal antibody to MyHC was obtained from the Developmental Studies. Hybridoma Bank was developed under the sponsorships of the NICHD and maintained by the University of Iowa. Publisher Copyright: © 2022 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.

PY - 2023/1

Y1 - 2023/1

N2 - Aim: It has been suggested that the proliferation and early differentiation of myoblasts are impaired in Marfan syndrome (MFS) mice during muscle regeneration. However, the underlying cellular and molecular mechanisms remain poorly understood. Here, we investigated muscle regeneration in MFS mouse models by analyzing the influence of the fibrotic niche on satellite cell function. Methods: In vivo, ex vivo, and in vitro experiments were performed. In addition, we evaluated the effect of the pharmacological inhibition of fibrosis using Ang-(1–7) on regenerating skeletal muscles of MFS mice. Results: The skeletal muscle of MFS mice shows an increased accumulation of collagen fibers (81.2%), number of fibroblasts (157.1%), and Smad2/3 signaling (110.5%), as well as an aberrant number of fibro-adipogenic progenitor cells in response to injury compared with wild-type mice. There was an increased number of proinflammatory and anti-inflammatory macrophages (3.6- and 3.1-fold, respectively) in regenerating muscles of wild-type mice, but not in the regenerating muscles of MFS mice. Our data show that proliferation and differentiation of satellite cells are altered (p ≤ 0.05) in MFS mice. Myoblast transplantation assay revealed that the regenerating muscles from MFS mice have reduced satellite cell self-renewal capacity (74.7%). In addition, we found that treatment with Ang-(1–7) reduces fibrosis (71.6%) and ameliorates satellite cell dysfunction (p ≤ 0.05) and muscle contractile function (p ≤ 0.05) in MFS mice. Conclusion: The fibrotic niche, caused by Fbn1 mutations, reduces the myogenic potential of satellite cells, affecting structural and functional muscle regeneration. In addition, the fibrosis inhibitor Ang-(1–7) partially counteracts satellite cell abnormalities and restores myofiber size and contractile force in regenerating muscles.

AB - Aim: It has been suggested that the proliferation and early differentiation of myoblasts are impaired in Marfan syndrome (MFS) mice during muscle regeneration. However, the underlying cellular and molecular mechanisms remain poorly understood. Here, we investigated muscle regeneration in MFS mouse models by analyzing the influence of the fibrotic niche on satellite cell function. Methods: In vivo, ex vivo, and in vitro experiments were performed. In addition, we evaluated the effect of the pharmacological inhibition of fibrosis using Ang-(1–7) on regenerating skeletal muscles of MFS mice. Results: The skeletal muscle of MFS mice shows an increased accumulation of collagen fibers (81.2%), number of fibroblasts (157.1%), and Smad2/3 signaling (110.5%), as well as an aberrant number of fibro-adipogenic progenitor cells in response to injury compared with wild-type mice. There was an increased number of proinflammatory and anti-inflammatory macrophages (3.6- and 3.1-fold, respectively) in regenerating muscles of wild-type mice, but not in the regenerating muscles of MFS mice. Our data show that proliferation and differentiation of satellite cells are altered (p ≤ 0.05) in MFS mice. Myoblast transplantation assay revealed that the regenerating muscles from MFS mice have reduced satellite cell self-renewal capacity (74.7%). In addition, we found that treatment with Ang-(1–7) reduces fibrosis (71.6%) and ameliorates satellite cell dysfunction (p ≤ 0.05) and muscle contractile function (p ≤ 0.05) in MFS mice. Conclusion: The fibrotic niche, caused by Fbn1 mutations, reduces the myogenic potential of satellite cells, affecting structural and functional muscle regeneration. In addition, the fibrosis inhibitor Ang-(1–7) partially counteracts satellite cell abnormalities and restores myofiber size and contractile force in regenerating muscles.

KW - Marfan syndrome

KW - angiotensin-1-7

KW - fibrosis

KW - satellite cell function

KW - skeletal muscle regeneration

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JO - Acta Physiologica

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ER -

The fibrotic niche impairs satellite cell function and muscle regeneration in mouse models of Marfan syndrome

Abstract

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Keywords

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