The extra sex combs product contains WD40 repeats and its time of action implies a role distinct from other Polycomb group products

Jeffrey Simon; Douglas Bornemann; Karen Lunde; Christopher Schwartz

doi:10.1016/0925-4773(95)00434-3

The extra sex combs product contains WD40 repeats and its time of action implies a role distinct from other Polycomb group products

Jeffrey Simon, Douglas Bornemann, Karen Lunde, Christopher Schwartz

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

The extra sex combs (esc) gene product is a transcriptional repressor of homeotic genes. Although it is classified in the Polycomb group (PcG) on the basis of phenotypic criteria, it is distinct from most other PcG repressors in its time of action during development. We describe the temporal profile of esc mRNA expression during embryogenesis and the stage-specific rescue of esc mutants with a heat shock-inducible esc cDNA transformation construct. Both experiments support the idea that esc product plays an early, transient role in repression of homeotic genes. We also present the sequence of a full-length esc cDNA. The predicted esc protein is composed primarily of multiple copies of a repeat motif, termed the WD40 repeat, which are likely used in protein-protein contact. We provide evidence that individual copies of the esc WD40 repeats are needed for function in vivo. We suggest that esc protein is an adaptor that binds to multiple protein partners and assists in the assembly or targeting of other PcG proteins.

Original language	English (US)
Pages (from-to)	197-208
Number of pages	12
Journal	Mechanisms of Development
Volume	53
Issue number	2
DOIs	https://doi.org/10.1016/0925-4773(95)00434-3
State	Published - Oct 1995

Bibliographical note

Funding Information:
We are grateful to Gary Struhl for providing esc genomic clones, mutant stocks, information about esc rescue constructs and advice. We thank Thomas Gutjahr and Markus No11f or sharing their genomic sequence of the esc gene prior to publication. We thank Madeline Serr and Tom Hays for the loan of a developmental Northern blot. We thank Ellen Miller for performing some of the DNA sequencing runs and Angela Brienzo and Maria Danos for generating some of the esc subclones used in this work. We thank Tom Hays and Ann Rougvie for discussions and critical comments on the manuscript. We also appreciate Ann’s help with late-night database searches. We thank Welcome Bender for providing a home for the initiation phase of this project. This work was supported by NSF grant IBN-9304936 and a University of Minnesota McKnight Land-Grant Professorship to J.S. D.B. was supportedi n part by NIH training grant GM07323.

Keywords

Anterior-posterior axis
Drosophila
Homeotic
Polycomb group
Repression
extra sex combs

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10.1016/0925-4773(95)00434-3

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title = "The extra sex combs product contains WD40 repeats and its time of action implies a role distinct from other Polycomb group products",

abstract = "The extra sex combs (esc) gene product is a transcriptional repressor of homeotic genes. Although it is classified in the Polycomb group (PcG) on the basis of phenotypic criteria, it is distinct from most other PcG repressors in its time of action during development. We describe the temporal profile of esc mRNA expression during embryogenesis and the stage-specific rescue of esc mutants with a heat shock-inducible esc cDNA transformation construct. Both experiments support the idea that esc product plays an early, transient role in repression of homeotic genes. We also present the sequence of a full-length esc cDNA. The predicted esc protein is composed primarily of multiple copies of a repeat motif, termed the WD40 repeat, which are likely used in protein-protein contact. We provide evidence that individual copies of the esc WD40 repeats are needed for function in vivo. We suggest that esc protein is an adaptor that binds to multiple protein partners and assists in the assembly or targeting of other PcG proteins.",

keywords = "Anterior-posterior axis, Drosophila, Homeotic, Polycomb group, Repression, extra sex combs",

author = "Jeffrey Simon and Douglas Bornemann and Karen Lunde and Christopher Schwartz",

note = "Funding Information: We are grateful to Gary Struhl for providing esc genomic clones, mutant stocks, information about esc rescue constructs and advice. We thank Thomas Gutjahr and Markus No11f or sharing their genomic sequence of the esc gene prior to publication. We thank Madeline Serr and Tom Hays for the loan of a developmental Northern blot. We thank Ellen Miller for performing some of the DNA sequencing runs and Angela Brienzo and Maria Danos for generating some of the esc subclones used in this work. We thank Tom Hays and Ann Rougvie for discussions and critical comments on the manuscript. We also appreciate Ann{\textquoteright}s help with late-night database searches. We thank Welcome Bender for providing a home for the initiation phase of this project. This work was supported by NSF grant IBN-9304936 and a University of Minnesota McKnight Land-Grant Professorship to J.S. D.B. was supportedi n part by NIH training grant GM07323.",

year = "1995",

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doi = "10.1016/0925-4773(95)00434-3",

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journal = "Mechanisms of Development",

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T1 - The extra sex combs product contains WD40 repeats and its time of action implies a role distinct from other Polycomb group products

AU - Simon, Jeffrey

AU - Bornemann, Douglas

AU - Lunde, Karen

AU - Schwartz, Christopher

N1 - Funding Information: We are grateful to Gary Struhl for providing esc genomic clones, mutant stocks, information about esc rescue constructs and advice. We thank Thomas Gutjahr and Markus No11f or sharing their genomic sequence of the esc gene prior to publication. We thank Madeline Serr and Tom Hays for the loan of a developmental Northern blot. We thank Ellen Miller for performing some of the DNA sequencing runs and Angela Brienzo and Maria Danos for generating some of the esc subclones used in this work. We thank Tom Hays and Ann Rougvie for discussions and critical comments on the manuscript. We also appreciate Ann’s help with late-night database searches. We thank Welcome Bender for providing a home for the initiation phase of this project. This work was supported by NSF grant IBN-9304936 and a University of Minnesota McKnight Land-Grant Professorship to J.S. D.B. was supportedi n part by NIH training grant GM07323.

PY - 1995/10

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N2 - The extra sex combs (esc) gene product is a transcriptional repressor of homeotic genes. Although it is classified in the Polycomb group (PcG) on the basis of phenotypic criteria, it is distinct from most other PcG repressors in its time of action during development. We describe the temporal profile of esc mRNA expression during embryogenesis and the stage-specific rescue of esc mutants with a heat shock-inducible esc cDNA transformation construct. Both experiments support the idea that esc product plays an early, transient role in repression of homeotic genes. We also present the sequence of a full-length esc cDNA. The predicted esc protein is composed primarily of multiple copies of a repeat motif, termed the WD40 repeat, which are likely used in protein-protein contact. We provide evidence that individual copies of the esc WD40 repeats are needed for function in vivo. We suggest that esc protein is an adaptor that binds to multiple protein partners and assists in the assembly or targeting of other PcG proteins.

AB - The extra sex combs (esc) gene product is a transcriptional repressor of homeotic genes. Although it is classified in the Polycomb group (PcG) on the basis of phenotypic criteria, it is distinct from most other PcG repressors in its time of action during development. We describe the temporal profile of esc mRNA expression during embryogenesis and the stage-specific rescue of esc mutants with a heat shock-inducible esc cDNA transformation construct. Both experiments support the idea that esc product plays an early, transient role in repression of homeotic genes. We also present the sequence of a full-length esc cDNA. The predicted esc protein is composed primarily of multiple copies of a repeat motif, termed the WD40 repeat, which are likely used in protein-protein contact. We provide evidence that individual copies of the esc WD40 repeats are needed for function in vivo. We suggest that esc protein is an adaptor that binds to multiple protein partners and assists in the assembly or targeting of other PcG proteins.

KW - Anterior-posterior axis

KW - Drosophila

KW - Homeotic

KW - Polycomb group

KW - Repression

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The extra sex combs product contains WD40 repeats and its time of action implies a role distinct from other Polycomb group products

Abstract

Bibliographical note

Keywords

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