The aim of these experiments was to determine the effects of combining high levels of vitamin E and lipoic acid supplementation on myocardial performance and arrhythmias during in vivo ischemia and reperfusion (I-R). Female Sprague-Dawley rats (4 months old) were randomly assigned to either a control diet group or a vitamin E and lipoic acid supplementation group. The control diet was made to AIN-93M standards which contains 75 IU vitamin E/kg diet. The supplemented diet contained 10,000 IU vitamin E/kg diet and 1.65 g lipoic acid/kg diet Following a 10-12 week feeding period, animals in each experimental group (control n=9; supplemented n=11) were subjected to an in vivo ischemia-reperfusion protocol. Animals were anesthetized, mechanically ventilated, and coronary occlusion was achieved by a ligature around the left coronary artery. Occlusion was maintained for 25 minutes followed by a 10 minute period of reperfusion. Cardiac rhythm disturbances were monitored via standard limb (lead II) ECG. Myocardial performance was analyzed by measuring 1) peak arterial pressure development and 2) the rate of change of pressure development by a catheter placed in the arch of the aorta. During ischemia-reperfusion, no significant differences (P>0.05) existed between control and supplemented animals in either ventricular arrhythmias or cardiac performance. These data suggest mat myocardial performance and rhythm disturbances are not affected by vitamin E and lipoic acid supplementation during in vivo ischemia-reperfusion in the rat heart.
|Original language||English (US)|
|State||Published - Dec 1 1997|