The effects of various antioxidants on the development of parthenogenetic porcine embryos

Hyung Soo Yuh, Dong Hun Yu, Mi Jung Shin, Hei Jung Kim, Ki Beom Bae, Dong Seok Lee, Hwi Cheul Lee, Won Kyong Chang, Soo Bong Park, Sang Gyu Lee, Hum Dai Park, Ji Hong Ha, Byung Hwa Hyun, Zae Young Ryoo

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The major objective of this study was to improve the development rate of parthenogenetic porcine embryos. In this study, the anti-oxidative and anti-apoptotic effects of three antioxidants, β-mercaptoethanol (β-ME), α-tocopherol, and extracellular superoxide dismutase (EC-SOD), were examined on the development of parthenogenetic porcine embryos. The development rate of parthenogenetic porcine embryos to the blastocyst stage was 8.1% for control; 19.1%, 14.6%, and 5.0% for 1, 3, and 5 μM β-ME; 17.2% and 17.5% for 50 and 100 μM α-tocopherol and 12.0% and 4.0% for EC-SOD transgenic mouse embryonic fibroblast (Tg-MEF) and EC-SOD non-transgenic mouse embryonic fibroblast (NTg-MEF) conditioned medium at day 3, respectively. Here, β-ME, α-tocopherol, and EC-SOD Tg-MEF conditioned medium increased the development rate of parthenogenetic porcine embryos to the blastocyst stage (P<0.05). The average number of total cells and apoptotic cells at the blastocyst was analyzed at the optimal conditions of the three antioxidants. The three antioxidants increased the average number of total cells at the blastocyst, and they decreased apoptotic cells at the blastocyst as compared to control without supplementation (P<0.05). When the reactive oxygen species levels in two-cell embryos after 1 μM β-ME and 100 μM α-tocopherol treatment were examined, those were lower than control group (P<0.05). In conclusion, it was found that the three antioxidants, β-mercaptoethanol, α-tocopherol, and EC-SOD Tg-MEF, conditioned medium can play a role as a strong stimulator in the development of parthenogenetic porcine embryos.

Original languageEnglish (US)
Pages (from-to)148-154
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume46
Issue number2
DOIs
StatePublished - Feb 2010
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgments This work was supported in part by Grant-in Aid from Rural Development Administration, Technology Development Program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea and Grant of the Korean Ministry of Education, Science, and Technology (The Regional Core Research Program).

Keywords

  • Antioxidant
  • Apoptosis
  • Embryo
  • Parthenogenesis
  • Porcine

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