The effects of subtherapeutic antibiotic use in farm animals on the proliferation and persistence of antibiotic resistance among soil bacteria

Sudeshna Ghosh, Timothy M LaPara

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

The use of antibiotics at subtherapeutic concentrations for agricultural applications is believed to be an important factor in the proliferation of antibiotic-resistant bacteria. The goal of this study was to determine if the application of manure onto agricultural land would result in the proliferation of antibiotic resistance among soil bacteria. Chlortetracycline-resistant bacteria were enumerated and characterized from soils exposed to the manure of animals fed subtherapeutic concentrations of antibiotics and compared to the chlortetracycline-resistant bacteria from soils at farms with restricted antibiotic use (dairy farms) and from non-agricultural soils. No significant differences were observed at nine different study sites with respect to the numbers and types of cultivated chlortetracycline-resistant bacteria. Genes encoding for tetracycline resistance were rarely detected in the resistant bacteria from these sites. In contrast, soils collected from a tenth farm, which allowed manure to indiscriminately accumulate outside the animal pen, had significantly higher chlortetracycline-resistance levels. These resistant bacteria frequently harbored one of 14 different genes encoding for tetracycline resistance, many of which (especially tet(A) and tet(L)) were detected in numerous different bacterial species. Subsequent bacterial enumerations at this site, following the cessation of farming activity, suggested that this farm remained a hotspot for antibiotic resistance. In conclusion, we speculate that excessive application of animal manure leads to the spread of resistance to soil bacteria (potentially by lateral gene transfer), which then serve as persistent reservoir of antibiotic resistance.

Original languageEnglish (US)
Pages (from-to)191-203
Number of pages13
JournalISME Journal
Volume1
Issue number3
DOIs
StatePublished - Jul 1 2007

Fingerprint

antibiotic resistance
Domestic Animals
soil bacteria
Microbial Drug Resistance
farmed animal species
antibiotics
chlortetracycline
Soil
persistence
Anti-Bacterial Agents
Bacteria
Chlortetracycline
animal manures
bacterium
Manure
bacteria
manure
soil
tetracycline
Tetracycline Resistance

Keywords

  • Antibiotic resistance
  • Chlortetracycline
  • Concentrated animal feeding operation
  • Lateral gene transfer
  • Subtherapeutic

Cite this

The effects of subtherapeutic antibiotic use in farm animals on the proliferation and persistence of antibiotic resistance among soil bacteria. / Ghosh, Sudeshna; LaPara, Timothy M.

In: ISME Journal, Vol. 1, No. 3, 01.07.2007, p. 191-203.

Research output: Contribution to journalArticle

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