The effects of sevoflurane on intracellular Ca2+ regulation in rat hepatocytes

Paul A. Iaizzo, Markus J. Seewald, Garth Powis, Russell A. Van Dyke

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The effects of sevoflurane, a new volatile anesthetic agent undergoing clinical trial, on the mobilization of intracellular Ca2+ in isolated rat hepatocytes was studied. This agent produced a dose-dependent release of 45Ca2+ from internal, non-mitochondrial stores of permeabilized hepatocytes (saponin treated). However, the administration of sevoflurane to aequorin-loaded intact hepatocytes had little or no effect on intracellular [Ca2+] (i.e., short transient or no increases in luminescence: no toxic effect). These data may indicate that because of the low solubility of sevoflurane, it has a selective effect on endoplasmic reticulum, i.e., mobilizing internal stores of Ca2+ relative to increasing transmembrane fluxes.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalToxicology Letters
Volume66
Issue number1
DOIs
StatePublished - Jan 1993

Keywords

  • Aequorin Ca transient
  • Ca release
  • Intracellular store
  • Isolated hepatocyte
  • Rat
  • Volatile anesthetic

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