The effects of aging and genotype on NMDA receptor expression in growth hormone receptor knockout (GHRKO) mice

Kathy Ruth Magnusson, Siba Ranjan Das, Daniel Kronemann, Andrzej Bartke, Peter R. Patrylo

    Research output: Contribution to journalArticlepeer-review

    5 Scopus citations

    Abstract

    Caloric restriction enhances N-methyl-d-aspartate (NMDA) receptor binding and upregulates messenger RNA expression of the GluN1 subunit during aging. Old growth hormone receptor knockout mice resemble old calorically restricted rodents in enhanced life span and brain function, as compared with aged controls. This study examined whether aged growth hormone receptor knockout mice also show enhanced expression of NMDA receptors. Six or 23- to 24-month-old male normal-sized control or dwarf growth hormone receptor knockout mice were assayed for NMDA-displaceable [3H]glutamate binding (autoradiography) and GluN1 subunit messenger RNA (in situ hybridization). There was slight sparing of NMDA receptor binding densities within aged medial prefrontal and motor cortices, similar to caloric restriction, but there were greater age-related declines in GluN1 messenger RNA in growth hormone receptor knockout versus control mice. These results suggest that some of the functional improvements in aged mice with altered growth hormone signaling may be due to enhancement of NMDA receptors, but not through the upregulation of messenger RNA for the GluN1 subunit.

    Original languageEnglish (US)
    Pages (from-to)607-619
    Number of pages13
    JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
    Volume66 A
    Issue number6
    DOIs
    StatePublished - Jun 2011

    Keywords

    • GluN1
    • Hippocampus
    • Laron mice
    • NMDA
    • Prefrontal cortex

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