The effect of the rate of cell proliferation on the synthesis of methotrexate poly-γ-glutamates in two human breast cancer cell lines

D. G. Kennedy, H. W. Van Den Berg, R. Clarke, R. F. Murphy

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The synthesis of methotrexate poly-γ-glutamates by the MDA-MB-436 and MCF-7 human breast cancer cell lines is highly dependent on the rate of cell growth. Slowly proliferating cells accumulate methotrexate to the same extent as rapidly proliferating cells but convert a lower percentage of the drug to polyglutamate forms. The longest polyglutamate-derivatives of methotrexate are generally only synthesized when the cells are doubling rapidly. The MDA-MB-436 cells exhibit a biphasic response of doubling time and polyglutamation to increasing initial cell number. Extremes of cell density are associated with long doubling times and reduced polyglutamate synthesis. MCF-7 cells show increasing doubling time and decreasing polyglutamate synthesis in response to increasing initial cell number.

Original languageEnglish (US)
Pages (from-to)3087-3090
Number of pages4
JournalBiochemical Pharmacology
Volume34
Issue number17
DOIs
StatePublished - Sep 1 1985
Externally publishedYes

Fingerprint Dive into the research topics of 'The effect of the rate of cell proliferation on the synthesis of methotrexate poly-γ-glutamates in two human breast cancer cell lines'. Together they form a unique fingerprint.

Cite this