The effect of substrates and inhibitors of cytochrome P-450 on the NADPH inhibition of the ATP-dependent, hepatic, microsomal calcium pump

Srivastava P. Srivastava, Neng Qian Chen, Jordan L. Holtzman

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Abstract

In hepatic microsomes one or more isozymes of cytochrome P-450 inhibits the ATP-dependent Ca2+ pump. This inhibition is reversible by GSH and appears to be due to a direct oxidation of the pump proteins by the oxygenated cytochrome. To determine which isozyme mediates this inhibiton, we have examined the effect of various substrates and inhibitors on the NADPH inhibition of Ca2+ uptake. We find that aminopyrine, benzphetamine and SKF-525A reverse this inhibition while a number of other substrates do not. This pattern suggests that a previously unreported isozyme of cytochrome P-450 mediates the Ca2+ pump inhibition.

Original languageEnglish (US)
Pages (from-to)135-137
Number of pages3
JournalFEBS Letters
Volume276
Issue number1-2
DOIs
StatePublished - Dec 10 1990

Bibliographical note

Funding Information:
AcknowledgementTs:h is studyw as supportedb y USPHS Grant ES 03731.

Keywords

  • Cytochrome P-450 inhibition
  • Microsomal Ca pump

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