TY - JOUR
T1 - The effect of radiopacifiers agents on pH, calcium release, radiopacity, and antimicrobial properties of different calcium hydroxide dressings
AU - Ordinola-Zapata, Ronald
AU - Bramante, Clovis Monteiro
AU - García-godoy, Franklin
AU - Moldauer, Bertram Ivan
AU - Minotti, Paloma Gagliardi
AU - Tercília Grizzo, Larissa
AU - Duarte, Marco Antonio Hungaro
N1 - Publisher Copyright:
© 2015 Wiley Periodicals, Inc.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - The aim of this study was to evaluate the antimicrobial activity, pH level, calcium ion release, and radiopacity of calcium hydroxide pastes associated with three radiopacifying agents (iodoform, zinc oxide, and barium sulfate). For the pH and calcium release tests, 45 acrylic teeth were utilized and immersed in ultrapure water. After 24 h, 72 h, and 7 days the solution was analyzed by using a pH meter and an atomic absorption spectrophotometer. Polyethylene tubes filled with the pastes were used to perform the radiopacity test. For the antimicrobial test, 25 dentin specimens were infected intraorally in order to induce the biofilm colonization and treated with the pastes for 7 days. The Live/Dead technique and a confocal microscope were used to obtain the ratio of live cells. Parametric and nonparametric statistical tests were performed to show differences among the groups (P<0.05). The pH analysis at 7 days showed significant differences (P<0.05) among the groups. No differences among the pastes were found in the calcium release test on the 7th day (P>0.05). The calcium hydroxide/iodoform samples had the highest radiopacity and antimicrobial activity against the biofilm-infected dentin in comparison to the other pastes (P<0.05). Calcium hydroxide mixed with 17% iodoform and 35% propylene glycol into a paste had the highest pH, calcium ion release, radiopacity, and the greatest antimicrobial action versus similar samples mixed with BaSO4 or ZnO.
AB - The aim of this study was to evaluate the antimicrobial activity, pH level, calcium ion release, and radiopacity of calcium hydroxide pastes associated with three radiopacifying agents (iodoform, zinc oxide, and barium sulfate). For the pH and calcium release tests, 45 acrylic teeth were utilized and immersed in ultrapure water. After 24 h, 72 h, and 7 days the solution was analyzed by using a pH meter and an atomic absorption spectrophotometer. Polyethylene tubes filled with the pastes were used to perform the radiopacity test. For the antimicrobial test, 25 dentin specimens were infected intraorally in order to induce the biofilm colonization and treated with the pastes for 7 days. The Live/Dead technique and a confocal microscope were used to obtain the ratio of live cells. Parametric and nonparametric statistical tests were performed to show differences among the groups (P<0.05). The pH analysis at 7 days showed significant differences (P<0.05) among the groups. No differences among the pastes were found in the calcium release test on the 7th day (P>0.05). The calcium hydroxide/iodoform samples had the highest radiopacity and antimicrobial activity against the biofilm-infected dentin in comparison to the other pastes (P<0.05). Calcium hydroxide mixed with 17% iodoform and 35% propylene glycol into a paste had the highest pH, calcium ion release, radiopacity, and the greatest antimicrobial action versus similar samples mixed with BaSO4 or ZnO.
KW - Biofilms
KW - Calcium hydroxide
KW - Confocal microscopy
KW - Dentistry
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U2 - 10.1002/jemt.22521
DO - 10.1002/jemt.22521
M3 - Article
C2 - 25990864
AN - SCOPUS:84931956184
SN - 1059-910X
VL - 78
SP - 620
EP - 625
JO - Microscopy Research and Technique
JF - Microscopy Research and Technique
IS - 7
ER -