The effect of propoxycaine·HCl on the physical properties of neuronal membranes

Jong Hwa Lee, Dong Il Kim, Hun Mun, Sung Key Lee, Jong Sun Park, Jong Ho Kim, Jang Hoon Lee, Yi Hoon Park, Young Chan Jeon, Ung Chan Yoon, Moon Kyung Bae, Hye Ock Jang, W. Gibson Wood, Il Yun

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Fluorescent probe techniques were used to evaluate the effect of propoxycaine·HCl on the physical properties (transbilayer asymmetric lateral and rotational mobilities, annular lipid fluidity and protein distribution) of synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortex. An experimental procedure was used based on selective quenching of both 1,3-di(1-pyrenyl)propane (Py-3-Py) and 1,6-diphenyl-1,3,5-hexatriene (DPH) by trinitrophenyl groups, and radiationless energy transfer (RET) from the tryptophans of membrane proteins to Py-3-Py. Propoxycaine·HCl increased the bulk lateral and rotational mobilities, and annular lipid fluidity in SPMVs lipid bilayers, and had a greater fluidizing effect on the inner monolayer than that of the outer monolayer. The magnitude of increasing effect on annular lipid fluidity in SPMVs lipid bilayer induced by propoxycaine·HCl was significantly far greater than magnitude of increasing effect of the drug on the lateral and rotational mobilities of SPMVs lipid bilayer. It also caused membrane proteins to cluster. These effects of propoxycaine·HCl on neuronal membranes may be responsible for some, though not all, of the local anesthetic actions of propoxycaine·HCl.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalChemistry and Physics of Lipids
Volume154
Issue number1
DOIs
StatePublished - Jul 2008

Bibliographical note

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

  • Annular lipid fluidity
  • Membrane protein clustering
  • Neuronal membranes
  • Propoxycaine·HCl
  • Transbilayer lateral and rotational mobilities

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