The effect of oral casein on hepatic glycogen metabolism in fasted rats

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Casein hydrolysate administration to fasted rats resulted in a biphasic response of glycogen synthase. Fifteen minutes after the protein meal, synthase R (active form) was increased. This was associated with a transient increase in hepatic glucose and glucose-6-phosphate (G6P) concentrations. Both glucose and G6P are known to stimulate synthase phosphatase activity, which would result in activation of synthase. Portal plasma insulin concentration was directly related to the amount of synthase R present. By 1 hour after the meal, synthase R activity was decreased compared with the control activity. Hepatic glycogen concentration was variable during the first 30 minutes after the meal, and then decreased progressively. Portal plasma glucagon concentration and phosphorylase a activity were elevated at all time points. The data suggest that the increased portal plasma glucagon concentration is the major hormonal signal for glycogen metabolism during the second hour following a pure protein meal. However, during the first 30 minutes glycogenolysis is attenuated, perhaps due to the transient increase in insulin and an increased intracellular G6P concentration.

Original languageEnglish (US)
Pages (from-to)649-653
Number of pages5
Issue number5
StatePublished - May 1993

Bibliographical note

Funding Information:
From the Metuholic Reseurch Lahoratov, C’eterun.s Administrution Medical Center, Minneapolis: and the Deparimrnts of Food Science and Nutrition and Medicine, Unrversitv of Minnesotu. Minneupolis, MN. Submitted February 19. 1992: accepted July 12. 1992. Supported by Merit Re\,tew Research Funds from the l,‘eteran.\ Administration. Address reprint requests to Muv C. Gunnon. PhD, Nutritional Biochemist, Director, Metabolic Research Lnboruto~ Medical Center, Minneapolis, MN 55417. Copytight c 1993 by W.B. Saunden C‘ompunv 00260495i93/4205-0019$03.00!0

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