The degree of equilibration of [3H]water across the cerebral capillary was evaluated by measuring its cerebral extraction fraction (E(w)) by using a dual label radioactive tracer technique. All tricyclic antidepressants (125 μmol/kg i.p. at 5 min) increased E(w) compared to base line. the rank order of the drugs in producing this response was doxepin>amitriptyline>imipramine>nortriptyline>desmethylimipramine>protriptyline. The effect of amitriptyline, the prototype tricyclic for this study, was rapid in onset (maximal effect within 5 min), reversible (duration 15 min), dose-dependent and generalized throughout the brain. Amitriptyline also induced a marked increase in the cerebral extracton fraction of [3H]ethanol. A difference in the time course of the drug effect on these two tracers indicated that the elevation in E was due to the increase in cerebral capillary permeability to both polar and lipid soluble substances. This work demonstrates that tricyclic antidepressants have important central effects on non-neuronal tissue.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Pharmacology and Experimental Therapeutics|
|State||Published - Jan 1 1980|