The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion

Omar Haque, Casie A. Pendexter, Benjamin T. Wilks, Ehab O.A. Hafiz, James F. Markmann, Korkut Uygun, Heidi Yeh, Shannon N. Tessier

Research output: Contribution to journalArticlepeer-review

Abstract

In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influence the number of donor blood cells retained in the allograft thereby exacerbating cold ischemic injury. We present the results of rat livers that underwent 24 h SCS after being flushed with a cold University of Wisconsin (UW) solution versus room temperature (RT) lactated ringers (LR) solution. These results were compared to livers that were not flushed prior to SCS and thoroughly flushed livers without SCS. We used viability and injury metrics collected during normothermic machine perfusion (NMP) and the number of retained peripheral cells (RPCs) measured by histology to compare outcomes. Compared to the cold UW flush group, livers flushed with RT LR had lower resistance, lactate, AST, and ALT at 6 h of NMP. The number of RPCs also had significant positive correlations with resistance, lactate, and potassium levels and a negative correlation with energy charge. In conclusion, livers exposed to cold UW flush prior to SCS appear to perform worse during NMP, compared to RT LR flush.

Original languageEnglish (US)
Article number23128
JournalScientific reports
Volume11
Issue number1
DOIs
StatePublished - Dec 2021

Bibliographical note

Funding Information:
This research was funded from the US National Institutes of Health (R01DK096075, R01DK114506, R01DK107875) and NSF ATP-Bio ERC grant (NSF 1941543). We gratefully acknowledge funding to SNT for Career Development from NIH (K99/R00 HL1431149), American Heart Association (18CDA34110049), Harvard Medical School Eleanor and Miles Shore Fellowship, and the Claflin Distinguished Scholar Award on behalf of the MGH Executive Committee on Research. Further, we thank the American Liver Foundation (2019 Hans Popper Memorial Postdoctoral Research Fellowship) and the American College of Surgeons (Grant number 1123-39991 scholarship endowment fund) for research support to Omar Haque. We also thank Florence Lin Min for the ATP analysis.

Publisher Copyright:
© 2021, The Author(s).

PubMed: MeSH publication types

  • Comparative Study
  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

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