The effect of agility exercise on eicosanoid excretion, oxidant status, and plasma lactate in dogs

Wendy I. Baltzer, Anna M. Firshman, Bernadette Stang, Jennifer J. Warnock, Elena Gorman, Erica C. McKenzie

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Background: The objective was to determine the effects of agility exercise on dogs of different skill levels with respect to urinary eicosanoids, urinary 15F2t-isoprostane (lipid peroxidation marker) and hematological/biochemical changes in plasma. Fifteen adult dogs had blood and urine samples obtained prior to, immediately and 4-hours following an agility exercise.Results: Hematocrit, red blood cells (RBC), albumin, and hemoglobin increased following exercise, with greatest increases correlating to increased skill group (novice, intermediate, masters); at 4-hours post-exercise, hematocrit, RBC, and hemoglobin were decreased. Phosphorus increased following exercise with the greatest increase in novice and intermediates. Plasma lactate increased 3.6-fold in masters, 3.2-fold in intermediates, and 1.2-fold in novice dogs. Urine thromboxane B2 (TXB2) more than tripled 4-hours post-exercise while 6-keto prostaglandin F (PGF1α, prostacyclin metabolite), prostaglandin E2 metabolites (13,14-dihydro-15-keto-prostaglandin A2 and 13,14-dihydro-15-keto-prostaglandin E2), and 13,14-dihydro-15-keto prostaglandin F were unaffected as determined by a competitive enzyme immunoassay and standardized by division with urine creatinine. Urine 15F2t-isoprostane increased insignificantly.Conclusions: Alterations in the plasma post-exercise were likely due to hemoconcentration from insensible water loss, splenic contraction and sympathetic stimulation while 4-hours later autohemodilution reduced RBC parameters. Elevations in plasma lactate and urinary TXB2 correlated with advanced skill level/speed of the dogs.

Original languageEnglish (US)
Article number249
JournalBMC Veterinary Research
Volume8
DOIs
StatePublished - Dec 28 2012

Keywords

  • Agility exercise
  • Dog
  • Oxidant stress
  • Plasma lactate
  • Thromboxane B2
  • Urinary eicosanoids
  • Urinary isoprostane

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