The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Thomas J. Gniadek; Michael T. Forman; Isabella Martin; Ravit Arav-Boger; Alexandra Valsamakis

doi:10.1016/j.diagmicrobio.2017.05.013

The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Thomas J. Gniadek
, Michael T. Forman
, Isabella Martin
, Ravit Arav-Boger
, Alexandra Valsamakis

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

Original language	English (US)
Pages (from-to)	40-43
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	89
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2017.05.013
State	Published - Sep 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Inc.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Aberrant real-time PCR
Adenovirus
Reduced fluorescence

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10.1016/j.diagmicrobio.2017.05.013

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abstract = "A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.",

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AU - Gniadek, Thomas J.

AU - Forman, Michael T.

AU - Martin, Isabella

AU - Arav-Boger, Ravit

AU - Valsamakis, Alexandra

PY - 2017/9

Y1 - 2017/9

N2 - A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

AB - A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

KW - Aberrant real-time PCR

KW - Adenovirus

KW - Reduced fluorescence

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UR - https://www.scopus.com/pages/publications/85020783915#tab=citedBy

U2 - 10.1016/j.diagmicrobio.2017.05.013

DO - 10.1016/j.diagmicrobio.2017.05.013

M3 - Article

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ER -

The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Abstract

Bibliographical note

UN SDGs

Keywords

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