The Drosophila Activin-like ligand Dawdle signals preferentially through one isoform of the Type-I receptor Baboon

Philip A. Jensen; Xiaoyan Zheng; Tzumin Lee; Michael B. O'Connor

doi:10.1016/j.mod.2009.09.003

The Drosophila Activin-like ligand Dawdle signals preferentially through one isoform of the Type-I receptor Baboon

Philip A. Jensen, Xiaoyan Zheng, Tzumin Lee, Michael B. O'Connor

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

How TGF-β-type ligands achieve signaling specificity during development is only partially understood. Here, we show that Dawdle, one of four Activin-type ligands in Drosophila, preferentially signals through Babo_c, one of three isoforms of the Activin Type-I receptor that are expressed during development. In cell culture, Dawdle signaling is active in the presence of the Type-II receptor Punt but not Wit, demonstrating that the Type-II receptor also contributes to the specificity of the signaling complex. During development, different larval tissues express unique combinations of these receptors, and ectopic expression of Babo_c in a tissue where it is not normally expressed at high levels can make that tissue sensitive to Dawdle signaling. These results reveal a mechanism by which distinct cell types can discriminate between different Activin-type signals during development as a result of differential expression of Type-I receptor isoforms.

Original language	English (US)
Pages (from-to)	950-957
Number of pages	8
Journal	Mechanisms of Development
Volume	126
Issue number	11-12
DOIs	https://doi.org/10.1016/j.mod.2009.09.003
State	Published - Dec 2009

Bibliographical note

Funding Information:
We thank members of the O’Connor lab for helpful discussions and A. Peterson for both technical support and comments on the manuscript. P.A.J. was funded, in part, by NIH predoctoral training grant T32 HD007430-11A1. T.L. and X.Z. were funded by NIH grant NS42049 to T.L. M.B.O. is an Investigator with the Howard Hughes Medical Institute.

Keywords

Activin
Baboon
Drosophila
Isoform
Specificity
TGF-β

Publisher link

10.1016/j.mod.2009.09.003

Find It

Find It at U of M Libraries

Cite this

@article{066980dfa6644360bc52e21794d3b4f6,

title = "The Drosophila Activin-like ligand Dawdle signals preferentially through one isoform of the Type-I receptor Baboon",

abstract = "How TGF-β-type ligands achieve signaling specificity during development is only partially understood. Here, we show that Dawdle, one of four Activin-type ligands in Drosophila, preferentially signals through Baboc, one of three isoforms of the Activin Type-I receptor that are expressed during development. In cell culture, Dawdle signaling is active in the presence of the Type-II receptor Punt but not Wit, demonstrating that the Type-II receptor also contributes to the specificity of the signaling complex. During development, different larval tissues express unique combinations of these receptors, and ectopic expression of Baboc in a tissue where it is not normally expressed at high levels can make that tissue sensitive to Dawdle signaling. These results reveal a mechanism by which distinct cell types can discriminate between different Activin-type signals during development as a result of differential expression of Type-I receptor isoforms.",

keywords = "Activin, Baboon, Drosophila, Isoform, Specificity, TGF-β",

author = "Jensen, {Philip A.} and Xiaoyan Zheng and Tzumin Lee and O'Connor, {Michael B.}",

note = "Funding Information: We thank members of the O{\textquoteright}Connor lab for helpful discussions and A. Peterson for both technical support and comments on the manuscript. P.A.J. was funded, in part, by NIH predoctoral training grant T32 HD007430-11A1. T.L. and X.Z. were funded by NIH grant NS42049 to T.L. M.B.O. is an Investigator with the Howard Hughes Medical Institute. ",

year = "2009",

month = dec,

doi = "10.1016/j.mod.2009.09.003",

language = "English (US)",

volume = "126",

pages = "950--957",

journal = "Mechanisms of Development",

issn = "0925-4773",

publisher = "John Wiley & Sons Inc.",

number = "11-12",

}

TY - JOUR

T1 - The Drosophila Activin-like ligand Dawdle signals preferentially through one isoform of the Type-I receptor Baboon

AU - Jensen, Philip A.

AU - Zheng, Xiaoyan

AU - Lee, Tzumin

AU - O'Connor, Michael B.

N1 - Funding Information: We thank members of the O’Connor lab for helpful discussions and A. Peterson for both technical support and comments on the manuscript. P.A.J. was funded, in part, by NIH predoctoral training grant T32 HD007430-11A1. T.L. and X.Z. were funded by NIH grant NS42049 to T.L. M.B.O. is an Investigator with the Howard Hughes Medical Institute.

PY - 2009/12

Y1 - 2009/12

N2 - How TGF-β-type ligands achieve signaling specificity during development is only partially understood. Here, we show that Dawdle, one of four Activin-type ligands in Drosophila, preferentially signals through Baboc, one of three isoforms of the Activin Type-I receptor that are expressed during development. In cell culture, Dawdle signaling is active in the presence of the Type-II receptor Punt but not Wit, demonstrating that the Type-II receptor also contributes to the specificity of the signaling complex. During development, different larval tissues express unique combinations of these receptors, and ectopic expression of Baboc in a tissue where it is not normally expressed at high levels can make that tissue sensitive to Dawdle signaling. These results reveal a mechanism by which distinct cell types can discriminate between different Activin-type signals during development as a result of differential expression of Type-I receptor isoforms.

AB - How TGF-β-type ligands achieve signaling specificity during development is only partially understood. Here, we show that Dawdle, one of four Activin-type ligands in Drosophila, preferentially signals through Baboc, one of three isoforms of the Activin Type-I receptor that are expressed during development. In cell culture, Dawdle signaling is active in the presence of the Type-II receptor Punt but not Wit, demonstrating that the Type-II receptor also contributes to the specificity of the signaling complex. During development, different larval tissues express unique combinations of these receptors, and ectopic expression of Baboc in a tissue where it is not normally expressed at high levels can make that tissue sensitive to Dawdle signaling. These results reveal a mechanism by which distinct cell types can discriminate between different Activin-type signals during development as a result of differential expression of Type-I receptor isoforms.

KW - Activin

KW - Baboon

KW - Drosophila

KW - Isoform

KW - Specificity

KW - TGF-β

UR - http://www.scopus.com/inward/record.url?scp=70350591046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350591046&partnerID=8YFLogxK

U2 - 10.1016/j.mod.2009.09.003

DO - 10.1016/j.mod.2009.09.003

M3 - Article

C2 - 19766717

AN - SCOPUS:70350591046

SN - 0925-4773

VL - 126

SP - 950

EP - 957

JO - Mechanisms of Development

JF - Mechanisms of Development

IS - 11-12

ER -

The Drosophila Activin-like ligand Dawdle signals preferentially through one isoform of the Type-I receptor Baboon

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this