Abstract
Procedures and solutions were developed for dissociating embryos of Blattella germanica in preparation for primary cell culture. Trypsin solutions were maximally effective at 0.01% for germ bands but higher concentrations, 0.05 to 0.1% were needed for embryos in later stages.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 141-146 |
| Number of pages | 6 |
| Journal | In Vitro - Plant |
| Volume | 12 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1976 |
Keywords
- cell culture
- dissociation
- insect
- pH
- trypsin