The detection of alkaline phosphatase using an electrochemical biosensor in a single-step approach

Joanne H. Wang, Kevin Wang, Brandon Bartling, Chung Chiun Liu

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0-300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.

Original languageEnglish (US)
Pages (from-to)8709-8721
Number of pages13
JournalSensors
Volume9
Issue number11
DOIs
StatePublished - Nov 2009

Keywords

  • Alkaline phosphatase
  • Biosensor
  • Electrochemical detection

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