The design of protein-based catalysts using semisynthetic methods

Mark D. Distefano, Hao Kuang, Dongfeng Qi, Mazhary Aram

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The combination of site-directed mutagenesis and chemical modification has resulted in the preparation of protein conjugates with new and useful properties. Proteins modified with metal-chelating groups are proving useful for mapping tertiary and quaternary interactions using the technique of affinity cleavage. The attachment of cofactors, including pyridoxal and pyridoxamine, has resulted in the preparation of semisynthetic transaminases that display enzyme-like properties, including enantioselectivity, substrate specificity and reaction-rate acceleration.

Original languageEnglish (US)
Pages (from-to)459-465
Number of pages7
JournalCurrent Opinion in Structural Biology
Volume8
Issue number4
DOIs
StatePublished - Aug 1998

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