The design and use of an optical mapping system for the study of intracardiac electrical signaling

Maneesh Shrivastav, Megan B. Ghai, Ashish Singal, Paul A Iaizzo

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Fluorescent optical mapping of electrically active cardiac tissues provides a unique method to examine the excitation wave dynamics of underlying action potentials. Such mapping can be viewed as a bridge between cellular level and organ systems physiology, e.g., by facilitating the development of advanced theoretical concepts of arrhythmia. We present the design and use of a high-speed, high-resolution optical mapping system composed entirely of "off the shelf" components. The electrical design integrates a 256 element photodiode array with a 16 bit data acquisition system. Proper grounding and shielding at various stages of the design reduce electromagnetic interference. Our mechanical design provides flexibility in terms of mounting positions and applications (use for whole heart or tissue preparations), while maintaining precise alignment between all optical components. The system software incorporates a user friendly graphical user interface, e.g., spatially recorded action potentials can be represented as intensity graphs or in strip chart format. Thus, this system is capable of displaying cardiac action potentials with high spatiotemporal resolution. Results from cardiac action potential mapping with intact mouse hearts are provided. It should be noted that this system could be readily configured to study isolated myocardial biopsies (e.g., isolated ventricular trabeculae). We describe the details of a versatile, user-friendly system that could be employed for a magnitude of study protocols.

Original languageEnglish (US)
Pages (from-to)138-151
Number of pages14
JournalIndian Pacing and Electrophysiology Journal
Volume12
Issue number4
DOIs
StatePublished - 2012

Keywords

  • Cardiac mapping
  • Optical mapping
  • Signal processing
  • Voltage sensitive dye

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