The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles

Alexander V. Zelenin, Andrey A. Alimov, Vladimir A. Barmintzev, Alexei O. Beniumov, Inessa A. Zelenina, Alexei M. Krasnov, Viktor A. Kolesnikov

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Fertilized eggs of loach (Misgurnus fossilis), rainbow trout (Salmo gairdneri) and zebrafish (Brachydanio rerio) were bombarded with high-velocity tungsten microprojectiles covered with plasmid DNA containing sequences of β-galactosidase and neomycin phosphotransferase genes. About 70% of the eggs survived the bombardment. The activity of both transferred genes was revealed in the fish developed from the bombarded eggs. Neomycin phosphotransferase gene sequences were detected by means of PCR amplification and Southern hybridization in the total DNA of zebrafish that survived after G418 treatment.

Original languageEnglish (US)
Pages (from-to)118-120
Number of pages3
JournalFEBS Letters
Volume287
Issue number1-2
DOIs
StatePublished - Aug 5 1991

Bibliographical note

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

  • Fish transformation
  • High-velocity mechanical DNA transfer
  • neo Gene
  • β-gal Gene

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