The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles

Alexander V. Zelenin; Andrey A. Alimov; Vladimir A. Barmintzev; Alexei O. Beniumov; Inessa A. Zelenina; Alexei M. Krasnov; Viktor A. Kolesnikov

doi:10.1016/0014-5793(91)80029-3

The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles

Alexander V. Zelenin, Andrey A. Alimov, Vladimir A. Barmintzev, Alexei O. Beniumov, Inessa A. Zelenina, Alexei M. Krasnov, Viktor A. Kolesnikov

Pulmonary, Allergy, Critical Care and Sleep Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Fertilized eggs of loach (Misgurnus fossilis), rainbow trout (Salmo gairdneri) and zebrafish (Brachydanio rerio) were bombarded with high-velocity tungsten microprojectiles covered with plasmid DNA containing sequences of β-galactosidase and neomycin phosphotransferase genes. About 70% of the eggs survived the bombardment. The activity of both transferred genes was revealed in the fish developed from the bombarded eggs. Neomycin phosphotransferase gene sequences were detected by means of PCR amplification and Southern hybridization in the total DNA of zebrafish that survived after G418 treatment.

Original language	English (US)
Pages (from-to)	118-120
Number of pages	3
Journal	FEBS Letters
Volume	287
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(91)80029-3
State	Published - Aug 5 1991

Keywords

Fish transformation
High-velocity mechanical DNA transfer
neo Gene
β-gal Gene

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10.1016/0014-5793(91)80029-3

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title = "The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles",

abstract = "Fertilized eggs of loach (Misgurnus fossilis), rainbow trout (Salmo gairdneri) and zebrafish (Brachydanio rerio) were bombarded with high-velocity tungsten microprojectiles covered with plasmid DNA containing sequences of β-galactosidase and neomycin phosphotransferase genes. About 70% of the eggs survived the bombardment. The activity of both transferred genes was revealed in the fish developed from the bombarded eggs. Neomycin phosphotransferase gene sequences were detected by means of PCR amplification and Southern hybridization in the total DNA of zebrafish that survived after G418 treatment.",

keywords = "Fish transformation, High-velocity mechanical DNA transfer, neo Gene, β-gal Gene",

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AU - Zelenin, Alexander V.

AU - Alimov, Andrey A.

AU - Barmintzev, Vladimir A.

AU - Beniumov, Alexei O.

AU - Zelenina, Inessa A.

AU - Krasnov, Alexei M.

AU - Kolesnikov, Viktor A.

PY - 1991/8/5

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N2 - Fertilized eggs of loach (Misgurnus fossilis), rainbow trout (Salmo gairdneri) and zebrafish (Brachydanio rerio) were bombarded with high-velocity tungsten microprojectiles covered with plasmid DNA containing sequences of β-galactosidase and neomycin phosphotransferase genes. About 70% of the eggs survived the bombardment. The activity of both transferred genes was revealed in the fish developed from the bombarded eggs. Neomycin phosphotransferase gene sequences were detected by means of PCR amplification and Southern hybridization in the total DNA of zebrafish that survived after G418 treatment.

AB - Fertilized eggs of loach (Misgurnus fossilis), rainbow trout (Salmo gairdneri) and zebrafish (Brachydanio rerio) were bombarded with high-velocity tungsten microprojectiles covered with plasmid DNA containing sequences of β-galactosidase and neomycin phosphotransferase genes. About 70% of the eggs survived the bombardment. The activity of both transferred genes was revealed in the fish developed from the bombarded eggs. Neomycin phosphotransferase gene sequences were detected by means of PCR amplification and Southern hybridization in the total DNA of zebrafish that survived after G418 treatment.

KW - Fish transformation

KW - High-velocity mechanical DNA transfer

KW - neo Gene

KW - β-gal Gene

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The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles

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