The current progress of CRISPR/Cas9 development in plants

Due to increasing human population, the food security provided by global food production is getting compromised to meet its demand. Certain techniques of mutagenesis pave the way by improving the yield of crops. An editing technique Clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9 (Cas9), serves the purpose. It involves certain site-specific nucleases (SSNs), creating a break on specific site in DNA. These breaks are then repaired via pathways as-non-homologous end joining (NHEJ) or homology-directed recombination (HDR). This advance method allows precise editing, creating definitive mutation and is now known to develop non-transgenic plants too. Thus, the chapter summarizes some of the latest progression in CRISPR/Cas9 and its use in the field of plant sciences, resolving issues and providing a scope in plant research.