Abstract
Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 Å resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.
Original language | English (US) |
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Article number | 10.1107/S2053230X23009536 |
Pages (from-to) | 295 |
Number of pages | 300 |
Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
Volume | 79 |
Issue number | Pt 12 |
DOIs | |
State | Published - Dec 1 2023 |
Keywords
- HUH endonucleases; HUH-tags; Rep domains; bioconjugation; crystal structure; smacoviruses; ssDNA.
PubMed: MeSH publication types
- Journal Article