The crystal structure of the human smacovirus 1 Rep domain

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Abstract

Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 Å resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.
Original languageEnglish (US)
Article number10.1107/S2053230X23009536
Pages (from-to)295
Number of pages300
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume79
Issue numberPt 12
DOIs
StatePublished - Dec 1 2023

Keywords

  • HUH endonucleases; HUH-tags; Rep domains; bioconjugation; crystal structure; smacoviruses; ssDNA.

PubMed: MeSH publication types

  • Journal Article

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