The Cre-loxP recombination-based reporter system for plant transcriptional expression studies

Toshiro Shigaki, Ravindranadha R. Vyzasatya, Ali B. Sivitz, John M. Ward, Heven Sze, Kendal D. Hirschi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To facilitate the characterization of plant genes, the Cre-loxP site-specific recombination system was adapted to make reporter vectors for plant expression studies. This system allows promoter fragments to be cloned into a small vector (univector) and subsequently recombined in vitro with binary vectors containing different reporter genes precisely at near-perfect efficiency. We have constructed univector-adapted vectors with three reporters, β-glucuronidase, luciferase, and green fluorescent protein, and a BASTA-resistance gene for selection of plant transformants. Expression in plants using the new system was validated by comparison to conventional reporter vectors. These new vectors are efficient and economical alternatives to the other plant reporter vectors currently available. The royalty-free Cre-loxP system serves as a platform for the future expansion of recombination-based cloning vectors for plant research.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalPlant molecular biology
Volume58
Issue number1
DOIs
StatePublished - May 2005

Fingerprint

Genetic Recombination
Plant Genes
Genetic Vectors
Glucuronidase
Green Fluorescent Proteins
Luciferases
Reporter Genes
site-specific recombination
genetic vectors
luciferase
green fluorescent protein
reporter genes
genes
promoter regions
Research

Keywords

  • Cre-loxP recombination
  • GFP
  • GUS
  • Gene expression
  • Luciferase
  • Promoter

Cite this

The Cre-loxP recombination-based reporter system for plant transcriptional expression studies. / Shigaki, Toshiro; Vyzasatya, Ravindranadha R.; Sivitz, Ali B.; Ward, John M.; Sze, Heven; Hirschi, Kendal D.

In: Plant molecular biology, Vol. 58, No. 1, 05.2005, p. 65-73.

Research output: Contribution to journalArticle

Shigaki, Toshiro ; Vyzasatya, Ravindranadha R. ; Sivitz, Ali B. ; Ward, John M. ; Sze, Heven ; Hirschi, Kendal D. / The Cre-loxP recombination-based reporter system for plant transcriptional expression studies. In: Plant molecular biology. 2005 ; Vol. 58, No. 1. pp. 65-73.
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