The COPI complex functions in nuclear envelope breakdown and is recruited by the nucleoporin Nup153

Jin Liu, Amy J Prunuske, Ammon M. Fager, Katharine S. Ullman

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Nuclear envelope breakdown is a critical step in the cell cycle of higher eukaryotes. Although integral membrane proteins associated with the nuclear membrane have been observed to disperse into the endoplasmic reticulum at mitosis, the mechanisms involved in this reorganization remain to be fully elucidated. Here, using Xenopus extracts, we report a role for the COPI coatomer complex in nuclear envelope breakdown, implicating vesiculation as an important step. We have found that a nuclear pore protein, Nup153, plays a critical role in directing COPI to the nuclear membrane at mitosis and that this event provides feedback to other aspects of nuclear disassembly. These results provide insight into how key steps in nuclear division are orchestrated.

Original languageEnglish (US)
Pages (from-to)487-498
Number of pages12
JournalDevelopmental Cell
Volume5
Issue number3
DOIs
StatePublished - Sep 1 2003

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Coat Protein Complex I
Nuclear Pore Complex Proteins
Nuclear Envelope
Membranes
Porins
Mitosis
Membrane Proteins
Cells
Cell Nucleus Division
Feedback
Nuclear Pore
Nuclear Proteins
Xenopus
Eukaryota
Endoplasmic Reticulum
Cell Cycle

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The COPI complex functions in nuclear envelope breakdown and is recruited by the nucleoporin Nup153. / Liu, Jin; Prunuske, Amy J; Fager, Ammon M.; Ullman, Katharine S.

In: Developmental Cell, Vol. 5, No. 3, 01.09.2003, p. 487-498.

Research output: Contribution to journalArticle

Liu, Jin ; Prunuske, Amy J ; Fager, Ammon M. ; Ullman, Katharine S. / The COPI complex functions in nuclear envelope breakdown and is recruited by the nucleoporin Nup153. In: Developmental Cell. 2003 ; Vol. 5, No. 3. pp. 487-498.
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