The cholinergic innervation of the adrenal gland and its relation to enkephalin and nitric oxide synthase

Hans Holgert, Katarina Aman, Costatino Cozzari, Boyd K. Hartman, Steven Brimijoin, Piers Emson, Menek Goldstein, Tomas Hokfelt

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Abstract

Using a monoclonal antibody against rat brain choline acetyltransferase (ChAT) the cholinergic innervation of the adult rat adrenal gland was visualized. Almost all ChAT-positive fibres contained nitric oxide synthase (NOS), whereas enkephalin (ENK) was exclusively found in ChAT fibres among adrenaline chromaffin cells. The ChAT/NOS/ENK fibres disappeared after immunological sympathectomy, indicating a preganglionic origin. ChAT was not found in the superficial peptide- and NOS containing fibre plexus in the adrenal cortex or in small or large intra-adrenal ganglion neurones under control conditions. Even after colchicine treatment only one single ChAT-positive small ganglion neurone was found. It is possible, therefore that some small intra-adrenal ganglion neurones, which express NOS- and VIP-like immunoreactivities, are noncholi-nergic, nonadrenergic neurones.

Original languageEnglish (US)
Pages (from-to)2576-2580
Number of pages5
JournalNeuroreport
Volume6
Issue number18
DOIs
StatePublished - Dec 1995

Keywords

  • Adrenaline
  • Choline acetyltransferase
  • Ganglion neurone
  • Immunological sympathectomy

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    Holgert, H., Aman, K., Cozzari, C., Hartman, B. K., Brimijoin, S., Emson, P., Goldstein, M., & Hokfelt, T. (1995). The cholinergic innervation of the adrenal gland and its relation to enkephalin and nitric oxide synthase. Neuroreport, 6(18), 2576-2580. https://doi.org/10.1097/00001756-199512150-00030