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The Chemoselective One-Step Alkylation and Isolation of Thiophosphorylated Cdk2 Substrates in the Presence of Native Cysteine
Sarah E. Lee
, Lucy M. Elphick
, Holger B. Kramer
, Alexandra M.E. Jones
, Emma S. Child
, Alexandra A. Anderson
,
Laurent Bonnac
, Natsuko Suwaki
, Benedikt M. Kessler
, Véronique Gouverneur
, David J. Mann
Research output
:
Contribution to journal
›
Article
›
peer-review
8
Scopus citations
Overview
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Keyphrases
Cyclin-dependent Kinase 2 (CDK2)
100%
Chemoselective
100%
PH Dependence
100%
Alkylation
100%
Chemical Genetics
100%
Cysteine
100%
Genetic Approach
50%
Signaling Pathway
50%
Protein Kinase
50%
Modified Proteins
50%
Protein Substrate
50%
Thiophosphate
50%
ATP Analogues
50%
Cyclohexyl
50%
S-alkylation
50%
Analog-sensitive Kinase
50%
Analog-sensitive
50%
Specific Substrate
50%
Kinase Substrate
50%
Biochemistry, Genetics and Molecular Biology
Alkylation
100%
Cysteine
100%
Chemical Genetics
66%
Protein Kinases
66%
Thiophosphate
33%
Genetic Approach
33%
Adenosine Triphosphate
33%
Phosphotransferase
33%
Kinase
33%
Protein Kinase A
33%