Mycobacterium bovis (M. bovis) is the pathogen of animals and humans that can replicate in the phagosomes of myeloid cells. Cytosolic detection of bacterial products plays a crucial role in initiating the innate immune response, including autophagy activation and interferon-β (IFN-β) release. Although IFN-β release and autophagy activation have been reported during mycobacterium infection, the mechanisms underlying remains poorly defined. Here, we demonstrated that IFN-β release increases in macrophages exposed to M. bovis and this requires the activation of the DNA sensor of interferon-γ inducible protein 204 (IFI204). Knockdown of the IFI204 in immortalized and primary murine macrophages blocked IFN-β production and autophagy marker LC3 expression. Thus, our results indicate that the IFI204 is an important sensor for innate immune responses of M. bovis infection.
Bibliographical noteFunding Information:
We thank China Agricultural University, for providing the BSL-3 Laboratories for experiments using M. bovis culture and infection. We thank Dr. Syed Zahid Ali Shah for his enthusiastic help in language modification of this manuscript. We also thank Dr. Ruichao Yue, Dr. Chaosi Li, and Dr. Fushan Shi for their valuable suggestions and critical reading of this manuscript. This work was supported by the MoSTRCUK international cooperation project (Project No. 2013DFG32500), National Natural Science Foundation of China (Project No.31572487), Funding of State Key Lab of Agrobiotechnology (Project No. 2012SKLAB06-14). 2015 CAU Foreign Experts Major Projects (Project No: 2012z018). High-end Foreign Experts Recruitment Program (Project No: GDW20151100036).
© 2017 Chunfa, Xin, Qiang, Sreevatsan, Yang, Zhao and Zhou.
- DNA sensor
- M. bovis